Copyright
©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Dec 26, 2025; 17(12): 110894
Published online Dec 26, 2025. doi: 10.4252/wjsc.v17.i12.110894
Published online Dec 26, 2025. doi: 10.4252/wjsc.v17.i12.110894
Isolation and characterization of CD73+CD39+CD146+ mesenchymal stem cell subset from bone marrow
Kathryn Martin, Francesca Gullo, Research & Development, Gift of Life Center for Cell and Gene Therapy, Boca Raton, FL 33487, United States
Author contributions: Martin K performed the research; Gullo F designed the research study.
Institutional review board statement: The project did not involve research involving human or animal in any form. All fresh materials were donated with institutional review board consent. All purchased materials were approved and verified by the vendor. All vendors have undergone institutional approval. The presented research was approved by all parties necessary through Gift of Life Marrow Registry and Gift of Life Center for Cell and Gene Therapy.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Additional data for each donor of for subset and mesenchymal stem cell phenotypic markers are available from the corresponding author at kmartin@giftoflife.org.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Kathryn Martin, Researcher, Research & Development, Gift of Life Center for Cell and Gene Therapy, 5901 Broken Sound Parkway NW, Boca Raton, FL 33487, United States. kattmart80@gmail.com
Received: June 18, 2025
Revised: July 18, 2025
Accepted: November 12, 2025
Published online: December 26, 2025
Processing time: 190 Days and 12.1 Hours
Revised: July 18, 2025
Accepted: November 12, 2025
Published online: December 26, 2025
Processing time: 190 Days and 12.1 Hours
Core Tip
Core Tip: In this study, the co-expression of CD73, CD39, and CD146 enabled the identification and enrichment of a mesenchymal stem cell subset with pericyte-like properties from bone marrow cultures. This subset was also found in mobilized blood of adult donors, which serve as a source for allogenic stem cell transplants. Since pericytes are known to play a vital role in maintaining hematopoiesis in vivo, we anticipate that using this subset in a co-culture system with hematopoietic stem cells could potentially lead, with further investigation, to methods for expanding hematopoietic stem cells and creating an inventory of products that are readily available for transplants.