Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jan 26, 2025; 17(1): 101485
Published online Jan 26, 2025. doi: 10.4252/wjsc.v17.i1.101485
Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model
Ok-Hyeon Kim, Hana Kang, Eun Seo Chang, Younghyun Lim, Young-Jin Seo, Hyun Jung Lee
Ok-Hyeon Kim, Hyun Jung Lee, Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University, Seoul 06974, South Korea
Hana Kang, Eun Seo Chang, Hyun Jung Lee, Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul 06974, South Korea
Younghyun Lim, Young-Jin Seo, Department of Life Science, Chung-Ang University, Seoul 06974, South Korea
Author contributions: Kim OH, Kang H, Chang ES, Lim Y, Seo YJ, and Lee H contributed to the methodology; Kim OH and Lee HJ contributed to the conceptualization and writing-original draft; Kim OH, Kang H, Chang ES, and Lim Y contributed to the formal analysis; Kim OH contributed to validation and investigation; Seo YJ and Lee HJ contributed to supervision; Lee HJ contributed to review & editing, project administration, and funding acquisition.
Supported by National Research Foundation of Korea, No. RS-2024-00409554, No. 2023R1A2C2006894, and No. 2021R1A6A3A01088243.
Institutional animal care and use committee statement: All experimental procedures including animals were reviewed and approved by the Institutional Animal Care Use Committee of Chung-Ang University (Approval ID: A2021023) and performed in accordance with the Guide for the Care and Use of Laboratory Animals, 8th edition (NIH publication No. 85-23, National Academy Press, Washington, DC, United States, revised 2011).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: All data generated or analyzed during this study are included in this published article and its supplementary information files.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hyun Jung Lee, PhD, Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University, 84 Heuksuk-ro, Dongjak-gu, Seoul 06974, South Korea. pluto38@cau.ac.kr
Received: September 16, 2024
Revised: November 11, 2024
Accepted: January 8, 2025
Published online: January 26, 2025
Processing time: 125 Days and 22 Hours
Core Tip

Core Tip: Clinical trials utilizing mesenchymal stromal cells (MSCs) have been on the rise, but several barriers to broader adoption of MSCs as therapeutics remain to be addressed. Our study presents compelling evidence demonstrating the remarkable enhancement of immunomodulatory capabilities in functional polymer-based MSCs through three-dimensional culture. We specifically applied these optimized MSCs to a brain inflammation model and found a prolonged and more potent anti-inflammatory effect in vivo compared to conventionally cultured MSCs. The polymer-based three-dimensional cultures we propose could be an easy tool for mass culture for clinical applications.