Yehya A, Azar J, Al-Fares M, Boeuf H, Abou-Kheir W, Zeineddine D, Hadadeh O. Cardiac differentiation is modulated by anti-apoptotic signals in murine embryonic stem cells. World J Stem Cells 2024; 16(5): 551-559 [PMID: 38817332 DOI: 10.4252/wjsc.v16.i5.551]
Corresponding Author of This Article
Ola Hadadeh, PhD, Associate Research Scientist, Department of Anatomy, Cell Biology and Physiological Sciences, American University of Beirut, Bliss Street-Hamra, Beirut 1107-2020, Lebanon. hadadeh.ola@gmail.com
Research Domain of This Article
Developmental Biology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. May 26, 2024; 16(5): 551-559 Published online May 26, 2024. doi: 10.4252/wjsc.v16.i5.551
Cardiac differentiation is modulated by anti-apoptotic signals in murine embryonic stem cells
Amani Yehya, Joseph Azar, Mohamad Al-Fares, Helene Boeuf, Wassim Abou-Kheir, Dana Zeineddine, Ola Hadadeh
Amani Yehya, Joseph Azar, Mohamad Al-Fares, Wassim Abou-Kheir, Ola Hadadeh, Department of Anatomy, Cell Biology and Physiological Sciences, American University of Beirut, Beirut 1107-2020, Lebanon
Helene Boeuf, Inserm, Biotis, U1026, University Bordeaux, Bordeaux F-33000, France
Dana Zeineddine, Rammal Rammal Lab, Physio-Toxicity Environmental Team, Faculty of Sciences, Lebanese University, Nabatieh 0000, Lebanon
Co-first authors: Amani Yehya and Joseph Azar.
Co-corresponding authors: Dana Zeineddine and Ola Hadadeh.
Author contributions: Yehya A and Azar J contributed equally to this work as co-first authors, and they wrote the manuscript and performed data analysis and interpretation; Al-Fares M performed initial lab work and participated in data collection; Boeuf H provided the genetically manipulated cell lines employed in this study; Abou-Kheir W, Zeineddine D, and Hadadeh O contributed as co-senior authors, and they conceived the project, managed and supervised the work, discussed the data, acquired the reagents, and corrected the manuscript draft; Zeineddine D and Hadadeh O contributed as co-corresponding authors, and they acquired the funding and reagents; and all authors have reviewed and approved the final manuscript.
Supported bythe National Council for Scientific Research in Lebanon, CNRS-L.
Institutional review board statement: This is to confirm that no patient samples were used in this research study.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ola Hadadeh, PhD, Associate Research Scientist, Department of Anatomy, Cell Biology and Physiological Sciences, American University of Beirut, Bliss Street-Hamra, Beirut 1107-2020, Lebanon. hadadeh.ola@gmail.com
Received: December 30, 2023 Revised: January 29, 2024 Accepted: April 1, 2024 Published online: May 26, 2024 Processing time: 145 Days and 10.2 Hours
Core Tip
Core Tip: Embryonic stem cells (ESCs) exhibit unique characteristics of self-renewal and pluripotency, allowing for their spontaneous differentiation into multiple cell lineages. Apoptotic signaling presents one of the crucial networks that influence embryonic development. In this study, the upregulation of anti-apoptotic genes B-cell lymphoma 2, Pim-2, and metallothionein-1 in mouse ESCs altered cardiac differentiation. This alteration was evidenced by a decreased size of embryoid bodies, a reduced number of beating cardiomyocytes, and an attenuated expression of cardiac markers. The study emphasizes the critical role of apoptosis in cardiac differentiation, providing insights into the regulatory impact of apoptosis on early differentiation pathways.
