Petinati N, Kapranov N, Davydova Y, Bigildeev A, Pshenichnikova O, Karpenko D, Drize N, Kuzmina L, Parovichnikova E, Savchenko V. Immunophenotypic characteristics of multipotent mesenchymal stromal cells that affect the efficacy of their use in the prevention of acute graft vs host disease. World J Stem Cells 2020; 12(11): 1377-1395 [PMID: 33312405 DOI: 10.4252/wjsc.v12.i11.1377]
Corresponding Author of This Article
Nina Drize, PhD, Professor, Laboratory for Physiology of Hematopoiesis, National Research Center for Hematology, Noviy Zykovskiy, 4, Moscow 125167, Russia. drize.n@blood.ru
Research Domain of This Article
Hematology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. Nov 26, 2020; 12(11): 1377-1395 Published online Nov 26, 2020. doi: 10.4252/wjsc.v12.i11.1377
Immunophenotypic characteristics of multipotent mesenchymal stromal cells that affect the efficacy of their use in the prevention of acute graft vs host disease
Nataliya Petinati, Nikolay Kapranov, Yulia Davydova, Alexey Bigildeev, Olesya Pshenichnikova, Dmitriy Karpenko, Nina Drize, Larisa Kuzmina, Elena Parovichnikova, Valeriy Savchenko
Nataliya Petinati, Alexey Bigildeev, Dmitriy Karpenko, Nina Drize, Laboratory for Physiology of Hematopoiesis, National Research Center for Hematology, Moscow 125167, Russia
Nikolay Kapranov, Yulia Davydova, Laboratory for Immunophenotyping of Blood and Bone Marrow Cells, National Research Center for Hematology, Moscow 125167, Russia
Olesya Pshenichnikova, Laboratory for Genetic Engineering, National Research Center for Hematology, Moscow 125167, Russia
Larisa Kuzmina, Elena Parovichnikova, Valeriy Savchenko, Hematopoiesis Depression and Bone Marrow Transplantation Department, National Research Center for Hematology, Moscow 125167, Russia
Author contributions: Petinati N, Drize N and Savchenko V designed and coordinated the study; Petinati N, Kapranov N, Davydova Y, Bigildeev A and Drize N performed the experiments, acquired and analyzed data; Kuzmina L and Parovichnikova E collected clinical data; Pshenichnikova O and Karpenko D performed the statistical analysis; Petinati N and Drize N wrote the manuscript; all authors approved the final version of the article.
Supported byRussian Foundation for Basic Research, No. 19-29-04023.
Institutional review board statement: The study was reviewed and approved by the National Research Center for Hematology, Moscow, Russia Institutional Review Board (Approval No. 140, 18 April 2019).
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Nina Drize, PhD, Professor, Laboratory for Physiology of Hematopoiesis, National Research Center for Hematology, Noviy Zykovskiy, 4, Moscow 125167, Russia. drize.n@blood.ru
Received: May 28, 2020 Peer-review started: May 28, 2020 First decision: July 30, 2020 Revised: July 31, 2020 Accepted: September 1, 2020 Article in press: September 1, 2020 Published online: November 26, 2020 Processing time: 178 Days and 1.9 Hours
Core Tip
Core Tip: An attempt was made to identify the main differences between multipotent mesenchymal stromal cells (MSCs) samples that are effective and those that are ineffective in preventing the development of acute graft vs host disease after allogeneic bone marrow transplantation. The mean fluorescence intensity of HLA-ABC, HLA-DR, CD105, and CD146 was shown to be significantly lower on the surface of samples that were ineffective for prophylaxis. Significant differences were revealed between effective and ineffective MSCs in terms of their responses to interaction with lymphocytes and stimulation by pro-inflammatory cytokines. The patterns observed here indicate a possible mechanism of the immunosuppressive action of these cells in clinical use.
