Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jun 26, 2021; 13(6): 645-658
Published online Jun 26, 2021. doi: 10.4252/wjsc.v13.i6.645
Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system
Joice Fülber, Fernanda R Agreste, Sarah R T Seidel, Eric D P Sotelo, Ângela P Barbosa, Yara M Michelacci, Raquel Y A Baccarin
Joice Fülber, Fernanda R Agreste, Sarah R T Seidel, Eric D P Sotelo, Ângela P Barbosa, Raquel Y A Baccarin, Departamento de Clínica Médica, Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05506-270, Brazil
Yara M Michelacci, Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04044-020, Brazil
Author contributions: Fülber J, Michelacci YM, and Baccarin RYA designed the study and wrote the manuscript; Baccarin RYA was responsible for obtaining funds; Fülber J, Agreste FR, Seidel SRT, Sotelo EDP, Barbosa AP collected tissue samples; Fülber J, Michelacci YM, and Baccarin RYA conducted the experimental analysis; Fülber J performed cell culture, chondrogenic differentiation of mesenchymal stem cells and all laboratory tests; all authors read and approved the final manuscript.
Supported by Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, No. 001.
Institutional review board statement: The study was reviewed and approved by Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP).
Institutional animal care and use committee statement: The present work was approved by the Committee for Ethics in Research of the University of São Paulo (CEUA/USP, 1143080617), and it was carried out in accordance with USP guidelines, ARRIVE guidelines, and the EC Directive 2010/63/EU for animal experiments (http://ec.europa.eu/environment/chemicals/Lab_animals/Legislation_en.htm).
Conflict-of-interest statement: Authors of this manuscript have no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Joice Fülber, PhD, Postdoc, Departamento de Clínica Médica, Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 Cidade Universitária, São Paulo 05506-270, Brazil. jfulber@usp.br
Received: February 5, 2021
Peer-review started: February 5, 2021
First decision: March 17, 2021
Revised: March 29, 2021
Accepted: June 4, 2021
Article in press: June 4, 2021
Published online: June 26, 2021
Processing time: 140 Days and 15.2 Hours
ARTICLE HIGHLIGHTS
Research background

Osteoarthritis is the main cause of economic loss in equine industry as its prompt lameness and limits or ends the equine athletic career. Fewer studies involve comparison tests of mesenchymal stem cells (MSCs) for the chondrogenic lineage in order to find a consistent and long-term therapeutic solution.

Research motivation

Nanoparticle’s technology applied in tridimensional cell culture was an innovative method, as it had not yet been studied in MSCs for chondrocyte differentiation.

Research objectives

To study the three sources of MSCs in order to find out which niche has the best commitment with the cartilage lineage, to use them in joint diseases in the future.

Research methods

We investigate the chondrogenic differentiation in vitro with tridimensional technology with cell exposure to the nanoparticles, evaluate and quantified the aggrecan, the main proteoglycan present in the hyaline cartilage, and identified aggrecan and keratan sulfate.

Research results

Cell culture from the three sources exhibited expression of MSC markers and, after chondrocytes differentiation, demonstrated aggrecan and keratan sulfate.

Research conclusions

MSCs derived from synovial fluid and adipose tissue cultured under three-dimensional technology system conditions demonstrated better biocompatibility, suggesting chondrogenic superiority.

Research perspectives

Future tests involve clinical application of MSCs in equine diseases and, in vitro and in vivo test based on the development of biomaterial in conjunction with the MSCs of synovial fluid for the evaluation of in vitro efficacy and in vivo safety.