Maurya DK, Doi C, Pyle M, Rachakatla RS, Davis D, Tamura M, Troyer D. Non-random tissue distribution of human naïve umbilical cord matrix stem cells. World J Stem Cells 2011; 3(4): 34-42 [PMID: 21607135 DOI: 10.4252/wjsc.v3.i4.34]
Corresponding Author of This Article
Masaaki Tamura, Associate Professor, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States. mtamura@vet.ksu.edu
Article-Type of This Article
Original Aritcle
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Dharmendra Kumar Maurya, Chiyo Doi, Marla Pyle, Raja Shekar Rachakatla, Masaaki Tamura, Deryl Troyer, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States
Duane Davis, Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, United States
Author contributions: Maurya DK, Doi C, Rachakatla RS and Tamura M were responsible for the study design, experimental work, data evaluation and analysis, and drafting the manuscript; Pyle M, Davis D and Troyer D were consulted extensively in the experimental design and interpretation of results, as well as in the preparation of the manuscript; Tamura M was the research supervisor and participated in the study design, assessment of the results and drafting the manuscript.
Supported by (in part) the Kansas State University (KSU) Terry C. Johnson Center for Basic Cancer Research, the KSU College of Veterinary Medicine Dean’s Fund, KSU Targeted Excellence, Kansas State Legislative Appropriation and NIH1R21CA135599
Correspondence to: Masaaki Tamura, Associate Professor, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States. mtamura@vet.ksu.edu
Telephone: +1-785-5324825 Fax: +1-785-5324557
Received: September 13, 2010 Revised: January 10, 2011 Accepted: January 17, 2011 Published online: April 26, 2011
Abstract
AIM: To determine the tissue and temporal distribution of human umbilical cord matrix stem (hUCMS) cells in severe combined immunodeficiency (SCID) mice.
METHODS: For studying the localization of hUCMS cells, tritiated thymidine-labeled hUCMS cells were injected in SCID mice and tissue distribution was quantitatively determined using a liquid scintillation counter at days 1, 3, 7 and 14. Furthermore, an immunofluorescence detection technique was employed in which anti-human mitochondrial antibody was used to identify hUCMS cells in mouse tissues. In order to visualize the distribution of transplanted hUCMS cells in H&E stained tissue sections, India Black ink 4415 was used to label the hUCMS cells.
RESULTS: When tritiated thymidine-labeled hUCMS cells were injected systemically (iv) in female SCID mice, the lung was the major site of accumulation at 24 h after transplantation. With time, the cells migrated to other tissues, and on day three, the spleen, stomach, and small and large intestines were the major accumulation sites. On day seven, a relatively large amount of radioactivity was detected in the adrenal gland, uterus, spleen, lung, and digestive tract. In addition, labeled cells had crossed the blood brain barrier by day 1.
CONCLUSION: These results indicate that peripherally injected hUCMS cells distribute quantitatively in a tissue-specific manner throughout the body.
