ST2 gene deficiency alleviates acute gastric injury in mice by modulating inflammation and epithelial cell death

Table 1 Primer sequences, melting temperatures, and expected amplicon sizes used for real-time quantitative reverse transcriptase polymerase chain reaction analysis

Gene name	Forward primer (5’-3’)	Reverse primer (5’-3’)	Temperature (°C)		Expected base pair size
			Forward	Reverse
IL-33	TCCTTGCTTGGCAGTATCCA	TGCTCAATGTGTCAACAGACG	68	69	52
Beclin-1	ATACTGTTCTGGGGGTTTGCG	GTCTCTCCTTTTTCCACCTCTTC	63	59	111
HMGB1	GGCGAGCATCCTGGCTTATC	GGCTGCTTGTCATCTGCTG	59	63	86
NLRP3	TGCTCTTCACTGCTATCAAGCCCT	ACAAGCCTTTGCTCCAGACCCTAT	65	65	85
Caspase-1	AGATGGCACATTTCCAGGAC	GATCCTCCAGCAGCAACTTC	68	70	221
Bcl-2	GTGGTGGAGGAACTCTTCAG	GTTCCACAAAGGCATCCCAG	70	70	205
Bax	ACACCTGAGCTGACCTTG	AGCCCATGATGGTTCTGATC	67	68	292
Caspase-3	AAATTCAAGGGACGGGTCAT	ATTGACACAATACACGGGATCTGT	66	70	180
NF-κB	GCCGTGGAGTACGACAA	CGGTTTCCCATTTAGTATGT	66	64	298
β-actin	AGCTGCGTTTTACACCCTTT	AAGCCATGCCAATGTTGTCT	56	56	81

IL-33: Interleukin-33; HMGB1: High mobility group box-1; NLRP3: NOD-like receptor family, pyrin domain containing 3; NF-κB: Nuclear factor kappa B.

Table 2 Correlation between systemic concentration of cytokines of interest and histological score

Cytokines	Histological score
IL-1β	r = 0.9711,b; P = 0.0011
TNF-α	r = 0.8831,a; P = 0.0201
IL-17	r = 0.765; P = 0.076
IFN-γ	r = 0.736; P = 0.096
IL-6	r = 0.9121,a; P = 0.0111

¹Significant values of Spearman’s rank correlation coefficients.

^aP < 0.05.

^bP < 0.001.

IL-1β: Interleukin-1β; TNF-α: Tumor necrosis factor-α; IL-17: Interleukin-17; IFN-γ: Interferon-γ; IL-6: Interleukin-6.