Yinchenhao decoction attenuates obstructive jaundice-induced liver injury and hepatocyte apoptosis by suppressing protein kinase RNA-like endoplasmic reticulum kinase-induced pathway

Table 1 Primer sequences used for quantitative real-time polymerase chain reaction for rat liver tissue

Gene	Forward primer (5’-3’)	Reverse primer (5’-3’)
β-actin	GTTACCAGGGCTGCCTTCTC	GGGTTTCCCGTTGATGACC
PERK	GTTCATGGAGAATGGCGCTT	GACACTTTGCACACCAGGTT
CHOP	ACACCACCACACCTGAAAGC	TGGACACTGTCTCAAAGGCG
GADD34	GTTTGCACGAGATCGAAGCC	CCCTGAAAGCAGGGGTAAGG
Bax	CCGAAATGTTTGCTGACG	AGCCGATCTCGAAGGAAGT
Bcl-2	ACCTGAATGACCACCTAGAGC	TCCGACTGAAGAGCGAAC

PERK: Endoplasmic reticulum-resident protein kinase; CHOP: CCAAT/enhancer-binding protein homologous protein; GADD34: Growth arrest- and DNA damage-inducible gene 34; Bcl-2: B cell lymphoma/leukemia-2 gene; Bax: Bcl-2 related X protein.

Table 2 Primer sequences used for quantitative real-time polymerase chain reaction for BCL-3A rat hepatocytes

Gene	Forward primer (5’-3’)	Reverse primer (5’-3’)
GADPH	GTTACCAGGGCTGCCTTCTC	GGGTTTCCCGTTGATGACC
PERK	GCTTGCTCCCACATCGGATA	CTAAGGACCTGCCGCGAG
CHOP	GATACTCGCTCTCCGCTCCA	CTTCCTTCTGGAACACTCTCTCC
GADD34	TTACCTGGACAGAAGCCAGC	AGTGCACCTTTCTACCCTTCAG
Bax	TTGCTACAGGGTTTCATCCAGG	CACTCGCTCAGCTTCTTGGT
Bcl-2	AGGATAACGGAGGCTGGGATG	CTCACTTGTGGCCCAGGTAT

GADPH: Glyceraldehyde 3-phosphate dehydrogenase; PERK: Endoplasmic reticulum-resident protein kinase; CHOP: CCAAT/enhancer-binding protein homologous protein; GADD34: Growth arrest- and DNA damage-inducible gene 34; Bcl-2: B cell lymphoma/leukemia-2 gene; Bax: B cell lymphoma/leukemia-2 gene related X protein.