Effect of fibulin-5 on adhesion, migration and invasion of hepatocellular carcinoma cells via an integrin-dependent mechanism

Table 1 Demographic features and clinicopathologic correlation of fibulin-5 in patients with hepatocellular carcinoma

Parameter		Total No. of patients(n = 140)	FBLN-5 expression		P value1
			High (n= 93)	Low (n = 47)
Age (yr)	< 50	54	40	14	0.211
	≥ 50	86	55	31
Gender	Male	113	79	34	0.287
	Female	27	16	11
Tumor size (cm)	< 5	82	57	25	0.618
	≥ 5	58	38	20
No. of tumor nodules	1	122	87	35	0.0232
	≥ 2	18	8	10
Recurrent Cancer	Absent	87	64	23	0.064
	Present	53	31	22
Cirrhosis	Absent	50	29	21	0.063
	Present	90	66	24
Serum AFP level	Negative	79	52	27	0.558
	Positive	61	43	18
HBV	HBsAg (-)	32	22	10	0.902
	HBsAg (+)	108	73	35
TNM tumor stage	I + II	102	79	23	0.0012
	III + IV	38	16	22
BCLC tumor stage	0 + A	93	74	19	0.0012
	B + C + D	47	21	26

¹P value from χ² or Fisher exact test;

²Statistically significant. HCC: Hepatocellular carcinoma; HBV: Hepatitis B virus; AFP: Alpha-fetoprotein; TNM: Tumor-node-metastasis; HBsAg: Hepatitis B surface antigen; FBLN-5: Fibulin-5; BCLC: Barcelona clinic liver cancer.

Table 2 Disease-specific survival by Cox regression analysis between the subgroups of various clincopathological parameters

Parameter		No.	No. of events	HR (95%CI)1	P value2
Age (yr)	< 50	54	18	0.373 (0.169-0.823)	0.158
	≥ 50	86	24
Gender	Male	113	30	0.864 (0.324-2.306)	0.771
	Female	27	12
Tumor size (cm)	< 5	82	23	1.874 (0.902-3.895)	0.093
	≥ 5	58	19
No. of tumor nodules	1	122	32	3.634 (1.481-8.916)	0.0053
	≥ 2	18	10
Recurrent cancer	Absent	87	15	0.587 (0.231-1.491)	0.263
	Present	53	28
Cirrhosis	Absent	50	14	0.924 (0.426-2.004)	0.841
	Present	90	29
Serum AFP level	Negative	79	21	0.615 (0.280-1.352)	0.227
	Positive	61	21
HBV	HBsAg (-)	32	11	1.487 (0.670-3.302)	0.329
	HBsAg (+)	108	31
TNM tumor stage	I + II	102	11	7.478 (2.571-21.753)	0.0013
	III + IV	38	31
BCLC tumor stage	0 + A	93	8	5.257 (1.707-16.186)	0.0043
	B + C + D	47	34
FBLN-5 Expression	High	95	13	4.276 (2.033-8.995)	0.0013
	Low	45	29

¹The 95% Wald Confidence Limits;

²Associations determined by Cox regression analyses;

³Statistically significant. AFP: Alpha-fetoprotein; HBV: Hepatitis B virus; TNM: Tumor-node-metastasis; BCLC: Barcelona clinic liver cancer; FBLN-5: Fibulin-5; HBsAg: Hepatitis B surface antigen.