CD97 promotes gastric cancer cell proliferation and invasion through exosome-mediated MAPK signaling pathway

Table 1 Four miRNA candidates and negative control sequences of CD97

Oligo	Sequence (5’-3’)
1F	TGCTGATGACATTCTGGATGGTGACCGTTTTGGCCACTGACTGACGGTCACCACAGAATGTCAT
1R	CCTGATGACATTCTGTGGTGACCGTCAGTCAGTGGCCAAAACGGTCACCATCCAGAATGTCATC
2F	TGCTGTATCTTCAAGGTTTGAGAGCAGTTTTGGCCACTGACTGACTGCTCTCACCTTGAAGATA
2R	CCTGTATCTTCAAGGTGAGAGCAGTCAGTCAGTGGCCAAAACTGCTCTCAAACCTTGAAGATAC
3F	TGCTGAAGAAAGTAGAGCTCCAGGCCGTTTTGGCCACTGACTGACGGCCTGGATCTACTTTCTT
3R	CCTGAAGAAAGTAGATCCAGGCCGTCAGTCAGTGGCCAAAACGGCCTGGAGCTCTACTTTCTTC
4F	TGCTGAAGATGAACAGGCCAAAGACCGTTTTGGCCACTGACTGACGGTCTTTGCTGTTCATCTT
4R	CCTGAAGATGAACAGCAAAGACCGTCAGTCAGTGGCCAAAACGGTCTTTGGCCTGTTCATCTTC
Negative control
Negative-F	TGCTGAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT
Negative-R	CCTGAAATGTACTGCGTGGAGACGTCAGTCAGTGGCCAAAACGTCTCCACGCGCAGTACATTTC

Table 2 Significant Kyoto encyclopedia of genes and genomes pathways enriched for target genes of differently expressed miRNAs

No.	Pathway ID	Pathway description	Target genes with pathway annotation, n (%)	P value
1	hsa05200	Pathways in cancer	179 (2.37)	5.50 ×10-3
2	hsa04060	Cytokine-cytokine receptor interaction	152 (2.01)	4.00 × 10-4
3	hsa04010:	MAPK signaling pathway	146 (1.93)	1.13 × 10-2
4	hsa04080	Neuroactive ligand-receptor interaction	139 (1.84)	1.81 × 10-2
5	hsa04510	Focal adhesion	121 (1.60)	2.00 × 10-4
6	hsa04810	Regulation of actin cytoskeleton	116 (1.54)	3.79 × 10-2
7	hsa04062	Chemokine signaling pathway	107 (1.42)	5.60 × 10-3
8	hsa04020	Calcium signaling pathway	95 (1.26)	5.88 × 10-2
9	hsa04142	Lysosome	72 (0.95)	2.3 × 10-3
10	hsa04670	Leukocyte transendothelial migration	68 (0.90)	2.36 × 10-3