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Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Oct 21, 2006; 12(39): 6343-6348
Published online Oct 21, 2006. doi: 10.3748/wjg.v12.i39.6343
Table 1 Clinical features of pancreatic cancer patients
CharacteristicPancreatic cancer patients
n = 61
Mean age ± SD (range) (yr)63 ± 10 (44-81)
Male/Female31 (50.8%)/30 (49.2%)
Primary tumor location
Head Body38 (62.3%) 15 (24.6%)
Tail8 (13.1%)
Smoking Yes/No18 (29.5%)/43 (70.5%)
Table 2 Primers and restriction enzymes used for genotyping analyses
GenePrimer (5’→ 3’; F = forward; R = reverse)Restrictionenzyme
UGT1A7 codon 129 and 131F1AAT TGC AGG AGT TTG aTT AA1VspI
F2AAT TGC AGG AGT TTG aTT A1VspI
R1TTC AGA GGC TAT TTC TAA GA
UGT1A7 codon 208F3ATG CTC GCT GGA CGG CAC CAT TGRsaI
R2TGC CGT GAC AGG GGT TTG GAG A
UGT1A7 allele specificF4ATT GCA GGA GTT TGT TTA AGG ACARsaI
R1TTC AGA GGC TAT TTC TAA GA
UGT1A9FCAGGTTTTGTGCTGGTATTTCTCCCA
RGCGGATATCCATAGGCACTGGCTTTCCCTGATGACA
ARPGGGCCGGGACTTGGAGGCGGTG
ICAGCACGCTCAGAGCCAG
Table 3 Allele and genotype frequencies of UGT1A7 and UGT1A9 gene polymorphisms in pancreatic cancers and controls n (%)
PolymorphismsCases (n = 61)Controls(n = 105)
UGT1A7 (exon 1)
Allele frequencies
*154 (44.3)74 (35.0)
*215 (12.3)46 (22.0)
*352 (42.6)89 (42.0)
*41 (0.8)1 (1.0)
CLUMP (T1): χ2 = 5.66 (df = 3), P = 0.10
CLUMP (T3-column used 2): χ2 = 4.75 (df = 1), Pc = 0.07 (uncorrected P = 0.03)
Genotype frequencies
*1*1 10 (16.4)14 (13.3)
*1*2  7 (11.5)12 (11.4)
*2*2  2 (3.3)6 (5.7)
*1*3 26 (42.6)34 (32.4)
*2*3  4 (6.6)22 (21.0)
*3*3 11 (18.0)16 (15.2)
*1*4  1 (1.6)0 (0.0)
*3*4 0 (0.0)1 (1.0)
CLUMP (T1): χ2 = 9.43 (df = 9), P = 0.21
CLUMP (T3-column used 5): χ2 = 6.05 (df = 1), Pc = 0.07 (uncorrected P = 0.01)
UGT1A9 (exon 1)
Allele frequencies
*1121 (99.2)206 (98.0)
*51 (0.8)2 (1.0)
*40 (0.0)2 (1.0)
CLUMP (T1): χ2 = 1.19 (df = 2), P = 0.79
Genotype frequencies
*1*160 (98.4)101 (96.2)
*1*51 (1.6)2 (1.9)
*1*40 (0.0)2 (1.9)
CLUMP (T1): χ2 = 1.19 (df = 5), P = 0.79
Table 4 Allele and genotype frequencies of ARP, SPINK1 and CFTR gene polymorphisms in pancreatic cancers and controls
PolymorphismsCases(n = 61)Controls(n = 105)
ARP
Allele frequencies
*14 (3.3)4 (2.0)
*24 (3.3)7 (3.0)
*3111 (91.0)195 (93.0)
*41 (0.8)0 (0.0)
*51 (0.8)2 (1.0)
*61 (0.8)0 (0.0)
*70 (0.0)2 (1.0)
CLUMP (T1): χ2 = 5.25 (df = 6), P = 0.52
Genotype frequencies
*1*34 (6.6)4 (3.8)
*2*34 (6.6)7 (6.7)
*3*350 (82.0)90 (85.7)
*3*41 (1.6)0 (0.0)
*3*51 (1.6)2 (1.9)
*3*61 (1.6)0 (0.0)
*3*70 (0.0)2 (1.9)
CLUMP (T1): χ2 = 5.29 (df = 27), P = 0.54
SPINK1 (exon 3)
Allele frequencies
wt117 (95.9)206 (98.0)
P55S4 (3.3)2 (1.0)
N34S1 (0.8)2 (1.0)
CLUMP (T1): χ2 = 2.36 (df = 2), P = 0.29
Genotype frequencies
wt/wt56 (91.8)101 (96.2)
wt/P55S4 (6.6)2 (1.9)
wt/N34S1 (1.6)2 (1.9)
CLUMP (T1): χ2 = 2.40 (df = 5), P = 0.30
CFTR
Allele frequencies
wt119 (97.5)206 (98.0)
∆F5083 (2.5)4 (2.0)
CLUMP (T1): χ2 = 0.11 (df = 1), P = 1.0
Genotype frequencies
wt/wt58 (95.1)101 (96.2)
wt/∆F5083 (4.9)4 (3.8)
CLUMP (T1): χ2 = 0.11 (df = 2), P = 1.0