Lack of association between UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms and pancreatic cancer in Italian patients

doi:10.3748/wjg.v12.i39.6343

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Oct 21, 2006 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2006; 12(39): 6343-6348
Published online Oct 21, 2006. doi: 10.3748/wjg.v12.i39.6343

Lack of association between UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms and pancreatic cancer in Italian patients

Ada Piepoli, Annamaria Gentile, Maria Rosa Valvano, Daniela Barana, Cristina Oliani, Rosa Cotugno, Michele Quitadamo, Angelo Andriulli, Francesco Perri

Ada Piepoli, Annamaria Gentile, Maria Rosa Valvano, Rosa Cotugno, Michele Quitadamo, Angelo Andriulli, Francesco Perri, Unit and Research Laboratory of Gastroenterology, “Casa Sollievo della Sofferenza”, Hospital, IRCCS, San Giovanni Rotondo, Italy

Daniela Barana, Cristina Oliani, Department of Medical Oncology, Hospital and University of Verona, Verona, Italy

Cristina Oliani, Oncology Unit, ULSS5 Ovest Vicentino, Verona, Italy

Supported by the Italian Ministry of Health, grant No. RC0402GA19

Correspondence to: Dr Ada Piepoli, Research Laboratory Department of Gastroenterology, “Casa Sollievo della Sofferenza” Hospital, IRCCS, San Giovanni Rotondo, Italy. a.piepoli@operapadrepio.it

Telephone: +39-882-416281 Fax: +39-882-411879

Received: March 29, 2006
Revised: September 8, 2006
Accepted: September 9, 2006
Published online: October 21, 2006

Abstract

AIM: To investigate simultaneously UGT1A7, UGT1A9, ARP, SPINK and CFTR genes to verify whether genetic polymorphisms predispose to the development of pancreatic cancer (PC).

METHODS: Genomic DNA of 61 pancreatic cancer patients and 105 healthy controls (HC) were analyzed. UGT1A7 genotyping was determined by PCR-RFLP analysis. Specific PCR and sequencing were used to analyze genetic variants of UGT1A9, ARP, SPINK1 and CFTR genes.

RESULTS: Four different alleles (*1: WT; *2: N129K and R131K; *3: N129K, R131K, and W208R; and *4: W208R) in UGT1A7 and three different alleles (*1: WT; *4: Y242X; and *5: D256N) in UGT1A9 were detected. All UGT1A polymorphisms were observed at similar frequency in PC patients and HC. Seven different alleles in ARP were found in PC patients and HC at similar frequency. The SPINK1 mutations N34S and P55S occurred in five PC patients with a prevalence (4.1%) not significantly different from that observed (2.0%) in HC. The only CFTRΔF508 mutation was recognized in three PC patients with a prevalence (4.9%) similar to HC.

CONCLUSION: UGT1A7, UGT1A9, ARP, SPINK1 and CFTR gene polymorphisms are not associated with PC in Italian patients.

Keywords: Pancreatic cancer; Genetic polymorphisms; Risk factors; Sporadic