Competitive inhibition of adherence of enterotoxigenic Escherichia coli, enteropathogenic Escherichia coli and Clostridium difficile to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027

Table 1 Effect of purified adhesin of B. ado 1027 on adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo

Group	Bacteria/cell
	ETEC	EPEC	C.difficile
Adhesin (0 μg/mL)	108.83 ± 16.34	110.27 ± 13.12	110.00 ± 16.91
Adhesin (1 μg/mL)	106.00 ± 14.46	107.77 ± 13.28	108.07 ± 14.54
Adhesin (5 μg/mL)	102.97 ± 7.65	104.20 ± 10.82	104.23 ± 9.62
Adhesin (10 μg/mL)	81.37 ± 7.18b	81.73 ± 9.01b	78.83 ± 6.23b
Adhesin (20 μg/mL)	41.23 ± 7.13b	42.37 ± 7.54b	38.77 ± 7.82b
Adhesin (30 μg/mL)	11.97 ± 4.42b	11.33 ± 4.35b	11.40 ± 4.20b
B. ado 1027	10.87 ± 3.22	10.07 ± 3.32	11.73 ± 3.66

^bP < 0.01 vs adhesin (0 µg/mL).

Table 2 Mean fluorescent intensity (MFI) of human intestinal epithelial cell with adherent ETEC, EPEC and C. difficile

Group	MFI
	ETEC	EPEC	C.difficile
Adhesin (0 μg/mL)	5.76 ± 0.43	5.26 ± 0.29	5.14 ± 0.21
Adhesin (1 μg/mL)	5.55 ± 0.44	5.06 ± 0.32	5.06 ± 0.14
Adhesin (5 μg/mL)	5.10 ± 0.24	4.83 ± 0.28	4.84 ± 0.15
Adhesin (10 μg/mL)	3.91 ± 0.20b	3.67 ± 0.35b	3.25 ± 0.32b
Adhesin (20 μg/mL)	2.23 ± 0.43b	2.21 ± 0.32b	2.19 ± 0.26b
Adhesin (30 μg/mL)	1.41 ± 0.23b	1.25 ± 0.18b	0.89 ± 0.14b
B. ado 1027	1.25 ± 0.23	0.98 ± 0.13	0.79 ± 0.19

^bP < 0.01 vs adhesin (0 µg/mL) group