Competitive inhibition of adherence of enterotoxigenic Escherichia coli, enteropathogenic Escherichia coli and Clostridium difficile to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027

doi:10.3748/wjg.v10.i11.1630

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 1, 2004; 10(11): 1630-1633
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1630

Competitive inhibition of adherence of enterotoxigenic Escherichia coli, enteropathogenic Escherichia coli and Clostridium difficile to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027

Shi-Shun Zhong, Zhen-Shu Zhang, Ji-De Wang, Zhuo-Sheng Lai, Qun-Ying Wang, Ling-Jia Pan, Yue-Xin Ren

Shi-Shun Zhong, Zhen-Shu Zhang, Ji-De Wang, Zhuo-Sheng Lai, Qun-Ying Wang, Ling-Jia Pan, Yue-Xin Ren, Chinese PLA Institute of Digestive Diseases, Nanfang Hospital, First military Medical University, Guangzhou 510515, Guangdong Province, China

Author contributions: All authors contributed equally to the work.

Supported by Natural Science Foundation of Guangdong Province, No.010621

Correspondence to: Dr. Shi-Shun Zhong, Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First military Medical University, Guangzhou 510515, Guangdong Province, China. zdoctor@sohu.com

Telephone: +86-20-61641530

Received: January 10, 2004
Revised: February 4, 2004
Accepted: February 18, 2004
Published online: June 1, 2004

Abstract

AIM: To observe competitive inhibition of adherence of enterotoxigenic Escherichia coli (ETEC), enteropathogenic Escherichia coli (EPEC) and Clostridium difficile (C. difficile) to intestinal epithelial cell line Lovo by purified adhesin of Bifidobacterium adolescentis 1027 (B. ado 1027).

METHODS: The binding of bacteria to intestinal epithelial cell line Lovo was counted by adhesion assay. The inhibition of adherence of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo by purified adhesin of B. ado 1027 was evaluated quantitatively by flow cytometry.

RESULTS: The purified adhesin at the concentration of 10 µg/mL, 20 µg/mL and 30 µg/mL except at 1 µg/mL and 5 µg/mL could inhibit significantly the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo. Moreover, we observed that a reduction in bacterial adhesion was occurred with increase in the concentration of adhesin, and MFI (Mean fluorescent intensity) was decreased with increase in the concentration of adhesin.

CONCLUSION: The purified adhesin of B. ado 1027 can inhibit the adhesion of ETEC, EPEC and C. difficile to intestinal epithelial cell line Lovo in a dose-dependent manner.

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