Plasma proteomic profiling of gastric cancer: Super minimally invasive surgery vs organ-resection on trauma immunity

Figure 1 Heatmap illustrating the differential expression of proteins in the super minimally invasive surgery and organ-resection surgery pre- and postoperative groups. A: Thirteen differentially expressed proteins generated by comparative analysis of the pre- and postoperative super minimally invasive surgery groups; B: Thirty-seven differentially expressed proteins generated by comparative analysis of the pre- and postoperative organ-resection surgery groups (P < 0.05). GEA: Postoperative period of super minimally invasive surgery; GEP: Preoperative period of super minimally invasive surgery; GSA: Postoperative period of organ-resection surgery; GSP: Preoperative period of organ-resection surgery.

Figure 2 Boxplot of the four differentially expressed proteins among the 13 differentially expressed proteins in the super minimally invasive surgery group. These differentially expressed proteins are endothelial nitric oxide synthase, calcitonin, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase1, platelet-derived growth factor C. NOS3: Nitric oxide synthase; CALCA: Calcitonin; ST3GAL1: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase1; PDGFC: Platelet-derived growth factor C; GEA: Postoperative period of super minimally invasive surgery; GEP: Preoperative period of super minimally invasive surgery; NPX: Normalized protein expression.

Figure 3 Boxplot of 29 differentially expressed proteins that were upregulated after organ-resection surgery. GSA: Postoperative period of organ-resection surgery; GSP: Preoperative period of organ-resection surgery; NPX: Normalized protein expression.

Figure 4 Boxplot of the nine differentially expressed proteins common to the organ-resection surgery and super minimally invasive surgery groups. GEA: Postoperative period of super minimally invasive surgery; GEP: Preoperative period of super minimally invasive surgery; GSA: Postoperative period of organ-resection surgery; GSP: Preoperative period of organ-resection surgery; NPX: Normalized protein expression.

Figure 5 Pre- and postoperative super minimally invasive surgery and organ-resection surgery. Three commonly upregulated differential expression proteins and the upregulation amplitude display diagram. CALCA: Calcitonin; PDGFC: Platelet-derived growth factor C; NOS3: Nitric oxide synthase 3; GEA: Postoperative period of super minimally invasive surgery; GEP: Preoperative period of super minimally invasive surgery; GSA: Postoperative period of organ-resection surgery; GSP: Preoperative period of organ-resection surgery.

Figure 6 Differential expression protein analysis of gastric cancer patients in the super minimally invasive surgery group and the organ-resection surgery group. A: Differential expression protein correlation heatmap; B: Gene Ontology term enrichment statistical bar chart; C: Kyoto Encyclopedia of Genes and Genomes path annotation diagram; D: Pathway enrichment dot plot. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.