Optimal liquid culture media for Helicobacter pylori: Strain-specific growth in different broth formulations

Figure 1 Growth analysis of nine Helicobacter pylori strains cultured in 10 different broths at average concentrations ranging from 1000 to 5000000 colony-forming unit/mL. The optical density was measured at 24, 48, and 72 hours, and the results for each media and the average for all media are shown. A: The average growth of each strain across all initial inoculum densities and all broth types; B: Time-dependent growth analysis of bacterial strains. Color intensity represents the optical density at 600 nm (OD₆₀₀), with darker blue indicating higher bacterial growth. BB: Brucella broth; BHI: Brain heart infusion broth; CB: Columbia broth; CMCB: Chopped meat carbohydrate broth; EB: Eugon broth; FAB: Fastidious anaerobe broth; ISB: Iso-Sensitest broth; LB: Luria Bertani broth low salt; MHB: Mueller-Hinton broth; TSB: Tryptic soy broth.

Figure 2 Performance analysis of 10 different media. A: Performance analysis of broths; B: Time-dependent performance analysis of broths. ^aP < 0.0001,^bP < 0.005, and ^cP < 0.05 compared to Brucella broth; C: Order of broth performance at each time point. BB: Brucella broth; BHI: Brain heart infusion broth; CB: Columbia broth; CMCB: Chopped meat carbohydrate broth; EB: Eugon broth; FAB: Fastidious anaerobe broth; ISB: Iso-Sensitest broth; LB: Luria Bertani broth low salt; MHB: Mueller-Hinton broth; TSB: Tryptic soy broth.