Correlation between rpoB gene mutation in Mycobacterium avium subspecies paratuberculosis and clinical rifabutin and rifampicin resistance for treatment of Crohn’s disease

Figure 1 Sequence alignment of 81 bp region in rpoB gene of MAP. The region overlaps the 81 bp rifamycin resistant determinant region within M. tuberculosis. For MAP, this sequence is harbored in the region amplified by primers Knight1/Knight2. Differentially expressed rpoB mutations are indicated for MAP strains 18 and UCF5-RIF16r. Base pairs and amino acids range from 1363-1443 and 456-481. Numbering of amino acids correspond to MAP strain K-10 beta subunit. Dots correspond to homologous bases and specific nucleotides are underlined in the control sequence. MAP strain 185 is excluded from diagram.