Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T

Figure 1 SL-174T treated with different concentrations of L-NAME. A: Control; B: 0.2 mmol/L; C: 0.4 mmol/L; D: 0.8 mmol/L; E: 1.0 mmol/L. Data are represented as mean±SD of triplicate determinations.

Figure 2 Invasion and migration of different groups of SL-174T cells (×200). A: Invasion of control group cells; B: 0.8 mmol/L L-NAME invasion group; C: migration of control group; D: 0.8 mmol/L L-NAME migration group.

Figure 3 A: Expression of MMP-2 mRNA in SL-174T cells treated with different concentrations of L-NAME. M: Marker; lane 1: control; lanes 2-5: SL-174T cells treated with 0.2, 0.4, 0.8, and 1.0 mmol/L of L-NAME, respectively. The experiments were carried out in triplicate. Data are expressed as mean±SD; B: Expression of TIMP-2 mRNA in SL-174T cells treated with different concentrations of L-NAME. M: Marker; lane 1: control; lanes 2-5: SL-174T cells treated with 0.2, 0.4, 0.8, and 1.0 mmol/L of L-NAME, respectively. The experiments were carried out in triplicate. Data are expressed as mean±SD.