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World J Gastroenterol. Apr 7, 2026; 32(13): 115710
Published online Apr 7, 2026. doi: 10.3748/wjg.v32.i13.115710
Published online Apr 7, 2026. doi: 10.3748/wjg.v32.i13.115710
MassARRAY-based KRAS and GNAS hotspot mutation analysis of cystic fluid enables accurate classification of pancreatic cystic lesions
Wen-Fei Diao, Ming Cui, Jin-Heng Xiao, Sen Yang, Qing-Yuan Zheng, Rui-Yuan Xu, Xian-Lin Han, Ya Hu, Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China
Wen-Fei Diao, Ming Cui, Jin-Heng Xiao, Sen Yang, Rui-Yuan Xu, Xian-Lin Han, Ya Hu, Key Laboratory of Research in Pancreatic Tumor, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Wen-Fei Diao, Ming Cui, Jin-Heng Xiao, Sen Yang, Rui-Yuan Xu, Ya Hu, National Infrastructures for Translational Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Wen-Fei Diao, Ming Cui, Jin-Heng Xiao, Sen Yang, Rui-Yuan Xu, Ya Hu, State Key Laboratory of Complex, Severe, and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Tian-Qi Chen, Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
Co-first authors: Wen-Fei Diao and Ming Cui.
Co-corresponding authors: Xian-Lin Han and Ya Hu.
Author contributions: Diao WF, Cui M and Chen TQ conceptualized and designed the study; Diao WF, Chen TQ, Xiao JH and Yang S collected the data and cystic fluids sample; Xu RY is responsible for data curation; Diao WF, Cui M and Zheng QY performed data analysis; Diao WF and Cui M prepared the manuscript; Hu Y and Han XL supervised the study and performed draft reviewing and editing. Since Diao WF and Cui M contributed equally for the study completion and manuscript writing, they were listed as co-first authors. Hu Y and Han XL were listed as co-correspondence as they contributed equally in study supervision and draft reviewing. All authors contributed to this study and approved the manuscript to be published.
Supported by Noncommunicable Chronic Diseases-National Science and Technology Major Project, No. 2025ZD0552402; Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences, No. 2023-I2M-2-002; National High-Level Hospital Clinical Research Funding, No. 2022-PUMCH-D-001; Peking Union Medical College Hospital Talent Cultivation Program, Category D, No. UHB12625; and Milstein Medical Asian American Partnership (MMAAP) Foundation.
Institutional review board statement: The study was approved by the Ethics Committee of Peking Union Medical College Hospital (No. I-23PJ1601), in accordance with the ethical standards of the Institutional Research Committee and with the Helsinki declaration.
Informed consent statement: Informed consents were obtained from all participants in the study.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
STROBE statement: The authors have read the STROBE Statement—checklist of items, and the manuscript was prepared and revised according to the STROBE Statement—checklist of items.
Data sharing statement: The data that support the findings of this study are available on request from the corresponding author Ya Hu, upon reasonable request at huya@pumch.cn.
Corresponding author: Ya Hu, MD, Professor, Department of General Surgery, Peking Union Medical College Hospital, No. 1 Shuaifuyuan, Dongcheng District, Beijing 100730, China.
Received: October 24, 2025
Revised: December 1, 2025
Accepted: February 3, 2026
Published online: April 7, 2026
Processing time: 154 Days and 20.1 Hours
Revised: December 1, 2025
Accepted: February 3, 2026
Published online: April 7, 2026
Processing time: 154 Days and 20.1 Hours
Core Tip
Core Tip: Pancreatic cystic lesions (PCLs) are highly heterogenous, which included benign, pre-malignant and malignant lesions. Current diagnostic approaches for PCLs exhibit limited efficacy and accuracy. The implementation of MassARRAY-based KRAS and GNAS mutation analysis of pancreatic cystic fluids improves the precise identification of mucinous cysts and intraductal papillary mucinous neoplasms, thereby providing critical support for clinical decision-making.