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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 7, 2025; 31(45): 112618
Published online Dec 7, 2025. doi: 10.3748/wjg.v31.i45.112618
Published online Dec 7, 2025. doi: 10.3748/wjg.v31.i45.112618
Dialister-driven succinate accumulation is associated with disease activity and postoperative recurrence in Crohn’s disease
Albert Boronat-Toscano, Diandra Monfort-Ferré, Irene Vañó-Segarra, Gemma Valldosera, Claudia Cepero, Brenno Astiarraga, Laura Clua-Ferré, Lidia Cabrinety, Carme Abadia de Barbarà, Alicia Moliné, Aleidis Caro, Sonia Fernández-Veledo, Joan Vendrell, Iris Ginés, Margarita Menacho, Carolina Serena, Hospital Universitari Joan XXIII, Institut d’Investigació Sanitària Pere Virgili, Tarragona 43007, Catalonia, Spain
Maria Isabel Queipo-Ortuño, Isaac Plaza-Andrade, Lucía Aranega-Martín, Daniel Castellano-Castillo, Medical Oncology Clinical Management Unit, Virgen Victoria Hospital FIMABIS, Biomed Res Lab, Campus Teatinos S-N, Málaga 29010, Andalucia, Spain
Maria Isabel Queipo-Ortuño, Unidad de Gestión Clínica de Endocrinología y Nutrición del Hospital Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga, Universidad de Málaga, Málaga 29010, Spain
Roger Suau, Laura Clua-Ferré, Josep Manyé, Inflammatory Bowel Diseases Research Group, Institut de Recerca Germans Trias i Pujol, Badalona 08916, Catalonia, Spain
Eugeni Domènech, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona 08916, Spain
Eugeni Domènech, Department of Medicine, Universitat Autònoma de Barcelona, Barcelona 08035, Spain
Eugeni Domènech, Josep Manyé, Carolina Serena, CIBER de Enfermedades hepaticas y digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid 08029, Spain
Jose Francisco Sánchez-Herrero, Robert Benaiges-Fernandez, Lauro Sumoy, Genòmica d’Alt Contingut i Bioinformàtica, Institut de Recerca Germans Trias i Pujol, Badalona 08916, Catalonia, Spain
Sonia Fernández-Veledo, Joan Vendrell, CIBER De Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto De Salud Carlos III, Madrid 08029, Spain
Iris Ginés, Carolina Serena, Departament of Biochemistry and Biotecnology, Universitat Rovira i Virgili, Tarragona 43007, Catalonia, Spain
Co-first authors: Albert Boronat-Toscano and Maria Isabel Queipo-Ortuño.
Author contributions: Boronat-Toscano A, Monfort-Ferré D, Suau R, Vañó-Segarra I, Clua-Ferré L, Astiarraga B, and Ginés I conducted the experiments; Valldosera G, Cepero C, Cabrinety L, Abadia de Barbarà C, Moliné A, Domènech E, Caro A, and Menacho M contributed to the selection of the study population and the processing of human samples; Queipo-Ortuño MI, Suau R, Plaza-Andrade I, Aranega-Martín L, Castellano-Castillo D, Benaiges-Fernandez R, Sánchez-Herrero JF, and Sumoy L performed microbiota experiments and data analysis; Queipo-Ortuño MI, Vañó-Segarra I, Benaiges-Fernandez R, Sánchez-Herrero JF, Sumoy L, and Serena C carried out the statistical analyses; Boronat-Toscano A, Monfort-Ferré D, Fernández-Veledo S, Vendrell J, Menacho M, and Serena C conceptualized the study, interpreted the data; Boronat-Toscano A and Serena C drafted the manuscript; Queipo-Ortuño MI, Sumoy L, Manyé J, and Serena C reviewed the manuscript; Serena C serves as the guarantor of this work.
Supported by the grant from I Clinical GETECCU_Galapagos (to Menacho M and Serena C); the grant from the Ministerio de Ciencia, Innovación y Universidades, PID2023-146315OB-I00 (to Manyé J and Serena C); the grant by the Instituto Salud Carlos III, PI20/00420 (to Manyé J) and PI22/01498 (to Domènech E and Sumoy L), co-funded by the European Union; and the grant, PI-AGAUR 2022-B00577 (to Boronat-Toscano A).
Institutional review board statement: This study was approved by the Ethics Committee of the Hospital Universitari Joan XXIII (Approval No. PI21/00011).
Conflict-of-interest statement: Fernández-Veledo S, Vendrell J, and Serena C are co-inventors of a patent (WO2019141780A1) licensed to SUCCIPRO S.L. In addition, Fernández-Veledo S and Vendrell J are co-founders of this company. The authors declare that these relationships have neither influenced nor biased the design, execution, or interpretation of the present work. All authors further declare that they have no other financial or personal relationships that could be perceived as potential conflicts of interest.
Data sharing statement: The 16S rRNA gene sequencing data generated in this study have been deposited in the NCBI Sequence Read Archive. Data from the HJ23 cohort are available under BioProject accession number PRJNA1250964 (http://www.ncbi.nlm.nih.gov/bioproject/1250964) and PRJNA1241201 (http://www.ncbi.nlm.nih.gov/bioproject/1241201) for the validation cohort at IGTP. These datasets will be made publicly available upon publication. Functional profiling results presented in the manuscript were obtained through computational analysis (PICRUSt2) of the HJ23 cohort 16S rRNA data and are therefore fully reproducible using the deposited sequences. Quantitative PCR data from PBMCs and circulating succinate levels from the HJ23 cohort are provided in Supplementary Table 3.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Carolina Serena, PhD, Head, Principal Investigator, Hospital Universitari Joan XXIII, Institut d’Investigació Sanitària Pere Virgili, C/Escorxador s/n, Tarragona 43007, Catalonia, Spain. carolina.serena@iispv.cat
Received: August 1, 2025
Revised: August 29, 2025
Accepted: October 17, 2025
Published online: December 7, 2025
Processing time: 124 Days and 20.8 Hours
Revised: August 29, 2025
Accepted: October 17, 2025
Published online: December 7, 2025
Processing time: 124 Days and 20.8 Hours
Core Tip
Core Tip: The study reported that circulating succinate, a microbial and host metabolite, are markedly elevated in patients with Crohn’s disease. Interestingly, we found that succinate strongly correlates with inflammatory activity and is linked to changes in the gut microbiota, including an increased abundance of the slow succinate consumer genera Dialister. Impor