©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2022; 28(15): 1536-1547
Published online Apr 21, 2022. doi: 10.3748/wjg.v28.i15.1536
Published online Apr 21, 2022. doi: 10.3748/wjg.v28.i15.1536
Establishing a rabbit model of perianal fistulizing Crohn’s disease
Shuang-Shuang Lu, Wen-Jia Liu, Qiu-Ya Niu, Chun-Yan Huo, Yu-Qing Cheng, En-Jing Wang, Rong-Nan Li, Fang-Fang Feng, Yi-Ming Cheng, Jin Huang, Gastroenterology Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China
Shuang-Shuang Lu, Wen-Jia Liu, Yu-Qing Cheng, Rong-Nan Li, Fang-Fang Feng, Yi-Ming Cheng, Jin Huang, Graduate School, Dalian Medical University, Dalian 116044, Liaoning Province, China
En-Jing Wang, Graduate School, Nanjing Medical University, Nanjing 210000, Jiangsu Province, China
Rong Liu, Jin Huang, Medical Statistics Center, Changzhou University, Changzhou 213000, Jiangsu Province, China
Author contributions: These authors contributed to this work. Huang J and Niu QY conceived the concept; Lu SS designed the method; Lu SS completed the entire study with the help of Liu WJ, Huo CY, Li RN, Wang EJ, Feng FF, Liu R and Cheng YM; the histological sections were completed with the help of Cheng YQ and Huo CY; endoscopy was performed by Huang J and Niu QY; Lu SS, Huang J and Liu WJ discussed the results and revised the manuscript.
Institutional review board statement: This study was approved by the Institutional Review Board of Changzhou University (No. 2019102510).
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals.
Conflict-of-interest statement: The authors have nothing to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Corresponding author: Jin Huang, PhD, Professor, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, No. 68 Gehu Road, Wujin District, Changzhou 213000, Jiangsu Province, China. hj042153@hotmail.com
Received: November 25, 2021
Peer-review started: November 25, 2021
First decision: January 11, 2022
Revised: January 18, 2022
Accepted: March 6, 2022
Article in press: March 6, 2022
Published online: April 21, 2022
Processing time: 140 Days and 21.6 Hours
Peer-review started: November 25, 2021
First decision: January 11, 2022
Revised: January 18, 2022
Accepted: March 6, 2022
Article in press: March 6, 2022
Published online: April 21, 2022
Processing time: 140 Days and 21.6 Hours
Core Tip
Core Tip: In this work, we improved the method of Crohn's disease (CD) colitis induction and successfully established a rabbit perianal fistula CD preclinical model, which was confirmed by endoscopy and pathology. The anatomy of this mid- to large-sized animal can simulate the human intestinal environment and tolerate examination and operation. This model may be used to assess perianal fistulizing CD treatments and their effectiveness.