Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 21, 2018; 24(11): 1196-1205
Published online Mar 21, 2018. doi: 10.3748/wjg.v24.i11.1196
NOD2- and disease-specific gene expression profiles of peripheral blood mononuclear cells from Crohn’s disease patients
Holger Schäffler, Maria Rohde, Sarah Rohde, Astrid Huth, Nicole Gittel, Hannes Hollborn, Dirk Koczan, Änne Glass, Georg Lamprecht, Robert Jaster
Holger Schäffler, Maria Rohde, Sarah Rohde, Astrid Huth, Nicole Gittel, Hannes Hollborn, Georg Lamprecht, Robert Jaster, Department of Medicine II, Division of Gastroenterology, Rostock University Medical Center, Rostock 18057, Germany
Dirk Koczan, Institute of Immunology, Rostock University Medical Center, Rostock 18057, Germany
Änne Glass, Institute for Biostatistics and Informatics in Medicine and Ageing Research, Rostock 18057, Germany
Author contributions: Schäffler H, Rohde S and Jaster R designed the study; Huth A, Schäffler H and Lamprecht G took responsibility for patient care and follow-up; Rohde M, Rohde S, Hollborn H, Jaster R and Koczan D (microarray studies) performed the experiments; Gittel N, Huth A and Schäffler H collected the samples and performed the clinical characterization of the patients; Glass Ä performed the biostatistics; all authors analyzed the data; and Schäffler H and Jaster R wrote the manuscript.
Supported by a grant from the Damp-Foundation (2016-04) to Schäffler H and Rohde S.
Institutional review board statement: The study was approved by the ethics board of the Medical Faculty of the University of Rostock (A 2015-0042). Written informed consent was obtained from each participant prior to enrollment.
Conflict-of-interest statement: The authors declare that there is no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Robert Jaster, MD, Academic Research, Professor, Senior Scientist, Department of Medicine II, Division of Gastroenterology, Rostock University Medical Center, E.-Heydemann-Str. 6, Rostock 18057, Germany. robert.jaster@med.uni-rostock.de
Telephone: +49-381-4947349 Fax: +49-381-4947482
Received: December 21, 2017
Peer-review started: December 21, 2017
First decision: January 18, 2018
Revised: January 29, 2018
Accepted: February 1, 2018
Article in press: February 1, 2018
Published online: March 21, 2018
Processing time: 84 Days and 22.4 Hours
Core Tip

Core tip: Peripheral blood mononuclear cells (PBMCs) are a useful tool to study peculiarities of the immune response in the context of Crohn’s disease (CD). Here, we investigated whether PBMCs from patients with CD, even at the stage of clinical remission, exhibit altered gene expression profiles after challenge with pathogen-associated molecular patterns and vitamin D. For TREM1, lysozyme and CLEC5A, disease-associated expression patterns, with higher transcript levels in patient-derived PBMCs, were observed. The two latter genes, along with four other transcripts, also showed NOD2-dependent expression profiles. TREM1 and CLEC5A may act with NOD2 in a regulatory network with a pathophysiological role in CD.