Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 28, 2016; 22(44): 9727-9733
Published online Nov 28, 2016. doi: 10.3748/wjg.v22.i44.9727
Infliximab does not increase colonic cancer risk associated to murine chronic colitis
Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Vincenzo Arena, Maria Emiliana Caristo, Andrea Poscia, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Alessandro Sgambato, Antonio Gasbarrini, Franco Scaldaferri
Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy
Vincenzo Arena, Institute of Pathology, Catholic University of Sacred Heart, 00168 Rome, Italy
Maria Emiliana Caristo, Experimental Center, Catholic University of Sacred Heart, 00168 Rome, Italy
Andrea Poscia, Institute of Hygiene, Catholic University of Sacred Heart, 00168 Rome, Italy
Alessandro Sgambato, Institute of General Pathology, Catholic University of Sacred Heart, 00168 Rome, Italy
Author contributions: Lopetuso LR did most of research and writing; Petito V performed samples processing and in vitro analysis; Arena V performed histological and tumor analysis; Zinicola T, Graziani C, Gerardi V assisted with writing; Caristo ME and Poscia A performed the statistical analysis; Cammarota G, Papa A and Cufino V assisted with the editing; Sgambato A, Gasbarrini A and Scaldaferri F conceived the original concept, assisted with the research and performed the critical revision of the manuscript; Lopetuso LR, Petito V, Gasbarrini A and Scaldaferri F contributed equally to this work.
Supported by Crohn’s and Colitis Foundation of America, Research Fellowship Award, No. CON125252 (to Lopetuso LR); European Crohn’s and Colitis Organization Grant (to Scaldaferri F); Società Italiana di Gastroenterologia prize (to Scaldaferri F).
Institutional review board statement: The study was reviewed and approved by the Catholic University Institutional Review Board.
Institutional animal care and use committee statement: All experiments were approved by the Local Ethics Committee for Animal Research Studies at the Catholic University of Rome (protocol number F42/2009).
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at francoscaldaferri@gmail.com.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, Largo Gemelli 8, 00168 Rome, Italy. francoscaldaferri@gmail.com
Telephone: +39-6-30156018 Fax: +39-6-30156018
Received: June 27, 2016
Peer-review started: June 27, 2016
First decision: July 29, 2016
Revised: August 23, 2016
Accepted: September 14, 2016
Article in press: September 14, 2016
Published online: November 28, 2016
Processing time: 152 Days and 11.1 Hours
Core Tip

Core tip: We report our results on the potential role of Infliximab on cancer progression in AOM-DSS murine model of colorectal cancer associated to chronic colitis. AOM/DSS model was induced in C57BL/6 mice. Mice were injected with Infliximab during each DSS cycle while control mice received saline. Mice were sacrificed at week 10 and colons were analyzed macroscopically and microscopically for number of cancers and degree of inflammation. MTT assay was performed on CT26 to evaluate the potential influence of Infliximab (IFX) role on metabolic activity and proliferation. This study demonstrates that beside its well-known healing capacity, IFX does not increase proliferative cancer cells ability and colorectal cancer risk in AOM-DSS model of tumor on chronic colitis.