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Zhou HC, Xu DZ, Wang XP, Zhang JX, Ying-Huang, Yan YP, Zhu Y, Jin BQ. Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes. World J Gastroenterol 2001; 7(4): 583-586 [PMID: 11819836 DOI: 10.3748/wjg.v7.i4.583]
Hong-Chao Zhou, De-Zhong Xu, Xue-Ping Wang, Jing-Xia Zhang, Ying-Huang, Yong-Ping Yan, Department of Epidemiology, the Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China
Yong Zhu, Bo-Quan Jin, Department of Immunology, the Fourth Military Medical University, Xi’an 710033, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Nature Science Foundation of China, No. 39800121
Correspondence to: Dr. Yong Zhu, Department of Immunology, the Fourth Military Medical University, Xi’an 710033, China. juliu@fmmu.edu.cn, Shaanxi, http: //www.fmmu.edu.cn
Received: February 13, 2001 Revised: March 5, 2001 Accepted: March 12, 2001 Published online: August 15, 2001
Abstract
AIM: To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).
METHODS: Utilizing the method of computer prediction followed by a 4 h 51Cr release assay confirmation.
RESULTS: The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide “ALAHGVRAL (core 150-158)”. The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis.
CONCLUSION: The peptide (core 150-158) is the candidate epitope recognized by HLA-A2 restricted CTL.
