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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.
World J Gastroenterol. Jun 21, 2026; 32(23): 117597
Published online Jun 21, 2026. doi: 10.3748/wjg.v32.i23.117597
Application of intraoperative laparoscopic ultrasound guidance combined with Sonazoid contrast in surgical treatment of colorectal liver metastasis
Wen-Tao He, Hua Chen, Hong Li, Yu-Lin Li, Hao-Ran Wu, Wen-Bin Duan, Yang Liu
Wen-Tao He, Hua Chen, Hong Li, Yu-Lin Li, Hao-Ran Wu, Yang Liu, Department of General Surgery, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), Changde 415000, Hunan Province, China
Wen-Bin Duan, Department of Hepatobiliary Surgery, Hunan Provincial People’s Hospital, Changsha 410000, Hunan Province, China
Author contributions: He WT designed the research and wrote the first manuscript; He WT, Chen H, Li H, Wu HR, Duan WB, and Li YL contributed to conceiving the research and analyzing data; Liu Y conducted the analysis and provided guidance for the research; all authors reviewed and approved the final manuscript.
Supported by Major Scientific Research Project of National Clinical Key Specialty, No. Z2023165.
Institutional review board statement: This study was approved by the Ethic Committee of The First People’s Hospital of Changde City (Approval No. 2024-104-02).
Informed consent statement: As the study used anonymous and pre-existing data, the requirement for the informed consent from patients was waived.
Conflict-of-interest statement: The authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
Corresponding author: Yang Liu, MD, Department of General Surgery, Changde Hospital, Xiangya School of Medicine, Central South University (The First People’s Hospital of Changde City), No. 388 Renming Road, Changde 415000, Hunan Province, China. 15873639390@163.com
Received: January 30, 2026
Revised: March 1, 2026
Accepted: March 17, 2026
Published online: June 21, 2026
Processing time: 128 Days and 19.2 Hours
Abstract
BACKGROUND

Contrast-enhanced ultrasound (CEUS) performed intraoperatively for colorectal cancer liver metastases can detect concealed liver metastases not identified during preoperative examinations or routine intraoperative ultrasound. This capability is critical for determining the appropriate surgical approach.

AIM

To explore the advantages of Sonazoid-CEUS (S-CEUS) over gray-scale ultrasound (GSUS) for tumor localization during laparoscopic ultrasound-guided microwave ablation (MWA) in colorectal liver metastases (CRLM) and to evaluate the real-time guidance role of S-CEUS in ablation therapy.

METHODS

We retrospectively reviewed data from 259 patients with CRLM who underwent MWA at the First People’s Hospital of Changde City between June 2020 and December 2023. Intraoperative target lesion localization was performed using GSUS or S-CEUS. Target lesion visibility was scored using a 5-point scale, and visibility differences between GSUS and S-CEUS were compared. The duration of the Kupffer phase for the target lesions was observed, and the effects of MWA performed during the Kupffer phase were evaluated.

RESULTS

Preoperative magnetic resonance imaging (MRI) identified 273 lesions in 259 patients, whereas intraoperative S-CEUS detected 281 lesions, all confirmed as liver metastases via needle biopsy. The target lesion visibility scores were 2.87 ± 1.09 during GSUS scanning and 4.07 ± 0.74 (arterial phase) and 4.31 ± 0.58 (Kupffer phase) during the S-CEUS. The visibility scores in both the arterial and Kupffer phases of S-CEUS were superior to those in GSUS (both P < 0.05). The Kupffer phase of target lesions lasted > 1 hour. In patients with multiple pre-known lesions, all lesions were localized with a single contrast agent injection. Three occult lesions undetected via preoperative imaging were identified during the Kupffer phase. All lesions underwent MWA in the Kupffer phase. Immediate reassessment with repeat Sonazoid injection 15 minutes post-ablation and enhanced MRI at 1 month postoperatively demonstrated complete ablation of all lesions.

CONCLUSION

During MWA for CRLM, target lesion visibility in both the arterial and Kupffer phases of S-CEUS was superior to that of GSUS, facilitating improved target lesion localization. The Kupffer phase of S-CEUS provided valuable real-time guidance for MWA.

Keywords: Colorectal cancer; Liver metastasis; Ultrasound guidance; Laparoscopy; Sonazoid contrast imaging

Core Tip: Advances in imaging technology have improved preoperative lesion localization and surgical planning by providing valuable visual references in the surgical treatment for liver metastases from colorectal cancer. However, strategies to better localize occult lesions intraoperatively following chemotherapy warrants further exploration. Sonazoid (Shizhuoan in Chinese) is a contrast agent taken up by Kupffer cells, which could facilitate further localization of hepatic lesions. However, reports on its intraoperative application in China remain limited. This study compared the visualization of target lesions during laparoscopic microwave ablation for colorectal liver metastases using Sonazoid-enhanced contrast-enhanced ultrasound vs gray-scale ultrasound.

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