Study of the effects of nesfatin-1 on gastric function in obese rats

doi:10.3748/wjg.v23.i16.2940

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World Journal of Gastroenterology

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World J Gastroenterol. Apr 28, 2017; 23(16): 2940-2947
Published online Apr 28, 2017. doi: 10.3748/wjg.v23.i16.2940

Study of the effects of nesfatin-1 on gastric function in obese rats

Guo-Tao Yang, Hong-Ying Zhao, Yu Kong, Ning-Ning Sun, Ai-Qin Dong

Guo-Tao Yang, Ai-Qin Dong, Department of Third Neurology, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China

Hong-Ying Zhao, Department of Elderly Internal Medicine, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China

Yu Kong, Department of Second Digestion, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China

Ning-Ning Sun, Department of First Digestion, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China

Supported by Cangzhou City Science and Technology Plan Projects, No. 151302138.

Institutional review board statement: This study was reviewed and approved by the Cangzhou Clinical Medical School of Hebei Medical University’s institutional review board statement.

Conflict-of-interest statement: No potential conflicts of interest relevant to this article were reported.

Data sharing statement: No additional data are available.

Correspondence to: Dr. Guo-Tao Yang, Department of Third Neurology, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China. yangguotaotao@yeah.net

Telephone: +86-317-2179510 Fax: +86-317-2179510

Received: December 1, 2016
Peer-review started: December 2, 2016
First decision: December 28, 2016
Revised: February 15, 2017
Accepted: March 2, 2017
Article in press: March 2, 2017
Published online: April 28, 2017
Processing time: 147 Days and 14.5 Hours

Abstract

AIM

To investigate the effects of nesfatin-1 on gastric function in obese rats.

METHODS

The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.

RESULTS

Body weight, body length, and Lee’s index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This in vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.

CONCLUSION

Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.

Keywords: Obesity; Nesfatin-1; H⁺/K⁺-ATPase

Core tip: The high-fat diet-induced obese rat model was used to study the effects of nesfatin-1 on gastric function. We found that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in a dose-dependent manner within a certain period of time. In addition, the expression of H⁺/K⁺ ATPase was down-regulated, which may explain the mechanisms of gastric acid secretion changes.