Potential protective effects of Clostridium butyricum on experimental gastric ulcers in mice

doi:10.3748/wjg.v21.i27.8340

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World Journal of Gastroenterology

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World J Gastroenterol. Jul 21, 2015; 21(27): 8340-8351
Published online Jul 21, 2015. doi: 10.3748/wjg.v21.i27.8340

Potential protective effects of Clostridium butyricum on experimental gastric ulcers in mice

Fang-Yan Wang, Jia-Ming Liu, Hai-Hua Luo, Ai-Hua Liu, Yong Jiang

Fang-Yan Wang, Hai-Hua Luo, Ai-Hua Liu, Yong Jiang, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Guangzhou 510515, Guangdong Province, China

Fang-Yan Wang, Hai-Hua Luo, Ai-Hua Liu, Yong Jiang, Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China, Key Laboratory of Proteomics of Guangdong Province, Guangzhou 510515, Guangdong Province, China

Fang-Yan Wang, Hai-Hua Luo, Ai-Hua Liu, Yong Jiang, Department of Pathophysiology, Southern Medical University, Guangzhou 510515, Guangdong Province, China

Fang-Yan Wang, Department of Pathophysiology, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China

Jia-Ming Liu, School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China

Author contributions: Wang FY and Jiang Y conceived and designed the experiments; Wang FY, Liu JM and Luo HH performed the experiments; Wang FY, Luo HH and Liu AH analyzed the data; Wang FY and Jiang Y wrote the paper.

Supported by grants from The National Key Basic Research (973) Program of China, No. 2010CB529704 (to Jiang Y); the National Natural Science Foundation of China, No.81030055, and No. 81372030 (to Jiang Y); the Guangdong Provincial Natural Science Foundation (S2013010014422) (to Liu A); and the Program of Wenzhou Sci-tech Museum, No. Y20130215 (to Wang FY).

Institutional animal care and use committee statement: All the animal procedures carried out in the present study were in accordance with the guidelines of the Animal Ethics Committee of Wenzhou Medical University.

Conflict-of-interest statement: The authors declare that no competing interests exist.

Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at jiang48231@163.com. Participants gave informed consent for data sharing.

Correspondence to: Yong Jiang, MD, PhD, Department of Pathophysiology, Southern Medical University, No. 1023 Shatai South Road, Guangzhou 510515, Guangdong Province, China. jiang48231@163.com

Telephone: +86-20-61648231 Fax: +86-20-61648231

Received: January 15, 2015
Peer-review started: January 16, 2015
First decision: April 13, 2015
Revised: April 20, 2015
Accepted: May 7, 2015
Article in press: May 7, 2015
Published online: July 21, 2015
Processing time: 188 Days and 4.9 Hours

Abstract

AIM: To investigate the effects of Clostridium butyricum (C. butyricum) on experimental gastric ulcers (GUs) induced by alcohol, restraint cold stress, or pyloric ligation in mice, respectively.

METHODS: One hundred and twenty mice were randomly allocated into three types of gastric ulcer models (n = 40 each), induced by alcohol, restraint cold stress, or pyloric ligation. In each GU model, 40 mice were allocated into four groups (n = 10 each): the sham control group; model group (GU induction without pretreatment); C. butyricum group (GU induction with C. butyricum pretreatment); and Omeprazole group (GU induction with Omeprazole pretreatment). The effects of C. butyricum were evaluated by examining the histological changes in the gastric mucosal erosion area, the activities of superoxide dismutase (SOD) and catalase (CAT), the level of malondialdehyde (MDA), and the contents of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, leukotriene B₄ (LTB₄) and 6-keto-PGF-1α (degradation product of PGI₂) in the gastric tissue.

RESULTS: Our data showed that C. butyricum significantly reduced the gastric mucosal injury area and ameliorated the pathological conditions of the gastric mucosa. C. butyricum not only minimized the decreases in activity of SOD and CAT, but also reduced the level of MDA in all three GU models used in this study. The accumulation of IL1-β, TNF-α and LBT₄ decreased, while 6-keto-PGF-1α increased with pretreatment by C. butyricum in all three GU models.

CONCLUSION: Our data demonstrated the protective effects of pretreatment with C. butyricum on anti-oxidation and anti-inflammation in different types of GU models in mice. Further studies are needed to explore its potential clinical benefits.

Keywords: Inflammation; Probiotics; Oxidative stress; Clostridium butyricum; Gastric ulcer

Core tip: In this study, we reported that the probiotic Clostridium butyricum (C. butyricum) pretreatment obviously attenuated gastric mucosal lesions induced by different stimulations. The oxidative stress- and inflammation-related parameters detected in this study showed that anti-oxidation and anti-inflammation participate in the underlying mechanism of C. butyricum protective effect on gastric mucosa. Our findings provide a potential protective method for the gastric mucosa and a novel application for C. butyricum in the clinic.