Hori T, Ohashi N, Chen F, Baine AMT, Gardner LB, Hata T, Uemoto S, Nguyen JH. Simple and reproducible hepatectomy in the mouse using the clip technique. World J Gastroenterol 2012; 18(22): 2767-2774 [PMID: 22719184 DOI: 10.3748/wjg.v18.i22.2767]
Corresponding Author of This Article
Tomohide Hori, PhD, MD, Department of Neuroscience, Mayo Clinic in Florida, 4500 San Pablo Road, Jacksonville, FL 32224, United States. hori.tomohide@mayo.edu
Article-Type of This Article
Original Article
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World J Gastroenterol. Jun 14, 2012; 18(22): 2767-2774 Published online Jun 14, 2012. doi: 10.3748/wjg.v18.i22.2767
Simple and reproducible hepatectomy in the mouse using the clip technique
Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T Baine, Lindsay B Gardner, Toshiyuki Hata, Shinji Uemoto, Justin H Nguyen
Tomohide Hori, Feng Chen, Ann-Marie T Baine, Lindsay B Gardner, Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, United States
Norifumi Ohashi, Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan
Tomohide Hori, Toshiyuki Hata, Shinji Uemoto, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan
Justin H Nguyen, Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, FL 32224, United States
Author contributions: Hori T, Ohashi N and Hata T performed the research; Hori T analyzed the data and wrote the paper; Chen F, Baine AMT and Gardner LB supported this research; Nguyen JH provided the originality of this technique; Uemoto S and Nguyen JH supervised the research.
Supported by Grants to Justin H Nguyen from the Deason Foundation, Sandra and Eugene Davenport, Mayo Clinic CD CRT-II and NIH R01NS051646-01A2; partial grant to Tomohide Hori from the Uehara Memorial Foundation, No. 200940051, Tokyo 171-0033, Japan
Correspondence to: Tomohide Hori, PhD, MD, Department of Neuroscience, Mayo Clinic in Florida, 4500 San Pablo Road, Jacksonville, FL 32224, United States. hori.tomohide@mayo.edu
Telephone: +1-904-9532449 Fax: +1-904-9537117
Received: June 28, 2011 Revised: August 25, 2011 Accepted: April 12, 2012 Published online: June 14, 2012
Abstract
AIM: To investigate the reliability of massive hepatectomy models by using clip techniques.
METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.
RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.
CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.
