Hori T, Ohashi N, Chen F, Baine AMT, Gardner LB, Hata T, Uemoto S, Nguyen JH. Simple and reproducible hepatectomy in the mouse using the clip technique. World J Gastroenterol 2012; 18(22): 2767-2774 [PMID: 22719184 DOI: 10.3748/wjg.v18.i22.2767]
Corresponding Author of This Article
Tomohide Hori, PhD, MD, Department of Neuroscience, Mayo Clinic in Florida, 4500 San Pablo Road, Jacksonville, FL 32224, United States. hori.tomohide@mayo.edu
Article-Type of This Article
Original Article
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World J Gastroenterol. Jun 14, 2012; 18(22): 2767-2774 Published online Jun 14, 2012. doi: 10.3748/wjg.v18.i22.2767
Simple and reproducible hepatectomy in the mouse using the clip technique
Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T Baine, Lindsay B Gardner, Toshiyuki Hata, Shinji Uemoto, Justin H Nguyen
Tomohide Hori, Feng Chen, Ann-Marie T Baine, Lindsay B Gardner, Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, United States
Norifumi Ohashi, Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan
Tomohide Hori, Toshiyuki Hata, Shinji Uemoto, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan
Justin H Nguyen, Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, FL 32224, United States
Author contributions: Hori T, Ohashi N and Hata T performed the research; Hori T analyzed the data and wrote the paper; Chen F, Baine AMT and Gardner LB supported this research; Nguyen JH provided the originality of this technique; Uemoto S and Nguyen JH supervised the research.
Supported by Grants to Justin H Nguyen from the Deason Foundation, Sandra and Eugene Davenport, Mayo Clinic CD CRT-II and NIH R01NS051646-01A2; partial grant to Tomohide Hori from the Uehara Memorial Foundation, No. 200940051, Tokyo 171-0033, Japan
Correspondence to: Tomohide Hori, PhD, MD, Department of Neuroscience, Mayo Clinic in Florida, 4500 San Pablo Road, Jacksonville, FL 32224, United States. hori.tomohide@mayo.edu
Telephone: +1-904-9532449 Fax: +1-904-9537117
Received: June 28, 2011 Revised: August 25, 2011 Accepted: April 12, 2012 Published online: June 14, 2012
Abstract
AIM: To investigate the reliability of massive hepatectomy models by using clip techniques.
METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.
RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.
CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.
Hori T, Ohashi N, Chen F, Baine AMT, Gardner LB, Hata T, Uemoto S, Nguyen JH. Simple and reproducible hepatectomy in the mouse using the clip technique. World J Gastroenterol 2012; 18(22): 2767-2774 [PMID: 22719184 DOI: 10.3748/wjg.v18.i22.2767]
