Polese L, Boetto R, De Franchis G, Angriman I, Porzionato A, Norberto L, Sturniolo GC, Macchi V, De Caro R, Merigliano S. B1a lymphocytes in the rectal mucosa of ulcerative colitis patients. World J Gastroenterol 2012; 18(2): 144-149 [PMID: 22253520 DOI: 10.3748/wjg.v18.i2.144]
Corresponding Author of This Article
Lino Polese, MD, PhD, Researcher in Surgery, Department of Surgical and Gastroenterological Sciences, 1st Surgical Clinic, University of Padova, Via Giustiniani 2, 35128 Padova, Italy. linopolese@hotmail.com
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jan 14, 2012; 18(2): 144-149 Published online Jan 14, 2012. doi: 10.3748/wjg.v18.i2.144
B1a lymphocytes in the rectal mucosa of ulcerative colitis patients
Lino Polese, Riccardo Boetto, Giuseppe De Franchis, Imerio Angriman, Andrea Porzionato, Lorenzo Norberto, Giacomo Carlo Sturniolo, Veronica Macchi, Raffaele De Caro, Stefano Merigliano
Lino Polese, Riccardo Boetto, Imerio Angriman, Lorenzo Norberto, Giacomo Carlo Sturniolo, Stefano Merigliano, Department of Surgical and Gastroenterological Sciences, University of Padova, Via Giustiniani 2, 35128 Padova , Italy
Giuseppe De Franchis, Department of Laboratory Medicine, Padova University Hospital, Via Giustiniani 2, 35128 Padova, Italy
Andrea Porzionato, Veronica Macchi, Raffaele De Caro, Department of Human Anatomy and Physiology, University of Padova, Via Gabelli 65, 35128 Padova, Italy
Author contributions: Polese L, De Franchis G, Angriman I and Sturniolo GC designed the research; Boetto R, De Franchis G, Norberto L, Macchi V and De Caro R performed the research; Polese L and Porzionato A analysed the data; Polese L, Merigliano S, De Franchis G and Porzionato A wrote the manuscript.
Correspondence to: Lino Polese, MD, PhD, Researcher in Surgery, Department of Surgical and Gastroenterological Sciences, 1st Surgical Clinic, University of Padova, Via Giustiniani 2, 35128 Padova, Italy. linopolese@hotmail.com
Telephone: +39-049-0587897 Fax: +39-049-8216514
Received: March 7, 2011 Revised: June 12, 2011 Accepted: June 19, 2011 Published online: January 14, 2012
Abstract
AIM: To assess B1a cell expression in the rectal mucosa of ulcerative colitis (UC) patients in comparison with healthy controls.
METHODS: Rectal mucosa biopsies were collected from 15 UC patients and 17 healthy controls. CD5+ B cells were analysed by three colour flow cytometry from rectal mucosal samples after mechanical disaggregation by Medimachine®. Immunohistochemical analysis of B and T lymphocytes was also performed. Correlations between, on the one hand, rectal B1a cell concentrations and, on the other, erythrocyte sedimentation rate and C-reactive protein levels and clinical, endoscopic and histological disease activity indices were evaluated.
RESULTS: Rectal B-lymphocyte (CD19+/CD45+) rate and concentration were higher in UC patients compared with those in healthy controls (47.85% ± 3.12% vs 26.10% ± 3.40%, P = 0.001 and 501 ± 91 cells/mm2vs 117 ± 18 cells/mm2, P < 0.001); Rectal B1a cell density (CD5+CD19+) was higher in UC patients than in healthy controls (85 ± 15 cells/mm2vs 31 ± 6.7 cells/mm2, P = 0.009). Rectal B1a cell (CD5/CD19+) rate correlated inversely with endoscopic classification (Rs = -0.637, P < 0.05).
CONCLUSION: B1a lymphocytes seem to be involved in the pathogenesis of UC, however, the role they play in its early phases and in disease activity, have yet to be defined.
