Brief Article
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World J Gastroenterol. Oct 7, 2010; 16(37): 4738-4746
Published online Oct 7, 2010. doi: 10.3748/wjg.v16.i37.4738
Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer
Jian-Feng Yi, Yu-Min Li, Tao Liu, Wen-Ting He, Xun Li, Wen-Ce Zhou, Shi-Liang Kang, Xiang-Ting Zeng, Jun-Qiang Zhang
Jian-Feng Yi, Shi-Liang Kang, Xiang-Ting Zeng, The First College of Clinical Medicine of Lanzhou University, Lanzhou 730000, Gansu Province, China
Yu-Min Li, Tao Liu, Jun-Qiang Zhang, Key Laboratory of Digestive System Tumors, Gansu Province, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China
Wen-Ting He, Xun Li, Wen-Ce Zhou, Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
Author contributions: Yi JF, Li YM, Liu T, He WT, Li X and Zhou WC designed the study; Yi JF, Kang SL, Zeng XT and Zhang JQ collected human subject blood samples, epidemiological data and performed the experiments; Yi JF performed the statistical analysis and wrote the paper; Li YM, Liu T and He WT wrote and revised the manuscript.
Supported by National Natural Science Foundation of China, No. 30870364
Correspondence to: Yu-Min Li, Professor, Key Laboratory of Digestive System Tumors, Gansu Province, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China. lym19621225@hotmail.com
Telephone: +86-931-8942744 Fax: +86-931-8942744
Received: May 14, 2010
Revised: July 15, 2010
Accepted: July 22, 2010
Published online: October 7, 2010
Abstract

AIM: To investigate the effects of superoxide dismutase (SOD) polymorphisms (rs4998557, rs4880), Helicobacter pylori (H. pylori) infection and environmental factors in gastric cancer (GC) and malignant potential of gastric precancerous lesions (GPL).

METHODS: Copper-zinc superoxide dismutase (SOD1, CuZn-SOD)-G7958A (rs4998557) and manganese superoxide dismutase (SOD2, Mn-SOD)-Val16Ala (rs4880) polymorphisms were genotyped by SNaPshot multiplex polymerase chain reaction (PCR) in 145 patients with GPL (87 cases of gastric ulcer, 33 cases of gastric polyps and 25 cases of atrophic gastritis), 140 patients with GC and 147 healthy controls. H. pylori infection was detected by immunoblotting analysis.

RESULTS: The SOD1-7958A allele was associated with a higher risk of gastric cancer [odds ratio (OR) = 3.01, 95% confidence intervals (95% CI): 1.83-4.95]. SOD2-16Ala/Val genotype was a risk factor for malignant potential of GPL (OR = 2.04, 95% CI: 1.19-3.49). SOD2-16Ala/- genotype increased the risk of gastric cancer (OR = 2.85, 95% CI: 1.66-4.89). SOD1-7958A/- genotype, SOD2-16Ala/- genotype, alcohol drinking, positive family history and type I H. pylori infection were associated with risk of gastric cancer, and there were additive interactions between the two genotypes and the other three risk factors. SOD2-16Ala/Val genotype and positive family history were associated with malignant potential of GPL and jointly contributed to a higher risk for malignant potential of GPL (OR = 7.71, 95% CI: 2.10-28.22). SOD1-7958A/- genotype and SOD2-16Ala/- genotype jointly contributed to a higher risk for gastric cancer (OR = 6.43, 95% CI: 3.20-12.91).

CONCLUSION: SOD1-7958A/- and SOD2-16Ala/-genotypes increase the risk of gastric cancer in Chinese Han population. SOD2-16Ala/-genotype is associated with malignant potential of GPL.

Keywords: Copper-zinc superoxide dismutase; Manganese superoxide dismutase; Gastric cancer; Gastric precancerous lesions; Gene polymorphisms; Interaction