Basic Research
Copyright ©2007 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Feb 14, 2007; 13(6): 882-888
Published online Feb 14, 2007. doi: 10.3748/wjg.v13.i6.882
Effect of BN52021 on NFκ-Bp65 expression in pancreatic tissues of rats with severe acute pancreatitis
Shi-Hai Xia, Dian-Chun Fang, Chun-Xiu Hu, Hui-Ying Bi, Yin-Zhi Yang, Yao Di
Shi-Hai Xia, Dian-Chun Fang, Chun-Xiu Hu, Hui-Ying Bi, Yin-Zhi Yang, Yao Di, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30300465
Correspondence to: Dr. Dian-Chun Fang, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. xshhcx@sina.com
Telephone: +86-23-68754124 Fax: +86-23-68754124
Received: October 22, 2006
Revised: November 23, 2006
Accepted: January 9, 2007
Published online: February 14, 2007
Abstract

AIM: To investigate dynamic changes and significance of expression of NF-κBp65 in pancreatic tissues of rats with severe acute pancreatitis (SAP), as well as BN52021 effects.

METHODS: Wistar male rats were randomly divided into negative control group (NC group, n = 60), SAP-model group (SAP group, n = 60), and BN52021-treated group (BN group, n = 60), and each of the above groups was respectively divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h) (n = 10). By RT-PCR and Western blot, NF-κBp65 mRNA and its protein expression in pancreatic tissues of rats were detected respectively.

RESULTS: The expression of NF-κBp65 mRNA dynamically changed in both SAP groups and BN groups. The mRNA level was higher in SAP groups than NC groups at 2 h, 3 h, 12 h, and 24 h after operation (P < 0.05), higher in BN groups than NC groups at all time points (P < 0.05), and higher in BN groups than SAP group at 1 h (P < 0.05). The NF-κBp65 protein level was higher in SAP groups than NC groups at 1 h, 3 h, and 6 h (P < 0.01), and 2 h, 12 h, and 24 h (P < 0.05), higher in BN groups than NC groups at all time points (P < 0.05), and lower in BN groups than SAP groups at 1 h, 3 h, and 6 h (P < 0.05).

CONCLUSION: The expression of NF-κBp65 in pancreatic tissues is dynamically changed and the changes play an important role in pathogenesis of SAP. BN52021 exerts therapeutic effects through reducing the expression level of NF-κBp65 protein in the early stage of SAP.

Keywords: BN52021; Pancreatitis; NF-κB; Signal transduction