Basic Research
Copyright ©2007 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Sep 14, 2007; 13(34): 4566-4573
Published online Sep 14, 2007. doi: 10.3748/wjg.v13.i34.4566
Preparation method of an ideal model of multiple organ injury of rat with severe acute pancreatitis
Xi-Ping Zhang, Qian Ye, Xin-Ge Jiang, Mei-Li Ma, Fei-Bo Zhu, Rui-Ping Zhang, Qi-Hui Cheng
Xi-Ping Zhang, Department of General Surgery, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
Qian Ye, Xin-Ge Jiang, Mei-Li Ma, Fei-Bo Zhu, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang Province, China
Rui-Ping Zhang, First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Qi-Hui Cheng, Department of Gynaecology and Obstetrics, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province, No. 2003C130; No. 2004C142; foundation project for medical science and technology of Zhejiang provinc, No. 2003B134; grave foundation project for technological and development of Hangzhou, No. 2003123B19; intensive foundation project for technology of Hangzhou, No. 2004Z006; foundation project for medical science and technology of Hangzhou, No. 2003A004; foundation project for technology of Hangzhou, No. 2005224
Correspondence to: Xi-Ping Zhang, MD, Department of General Surgery, Hangzhou First People’s Hospital, 261 Huansha Road, Hangzhou 310006, Zhejiang Province, China. zxp99688@vip.163.com
Telephone: +86-571-87065701 Fax: +86-571-87914773
Received: May 10, 2007
Revised: June 20, 2007
Accepted: June 23, 2007
Published online: September 14, 2007
Abstract

AIM: To establish an ideal model of multiple organ injury of rats with severe acute pancreatitis (SAP).

METHODS: SAP models were induced by retrograde injection of 0.1 mL/100 g 3.5% sodium taurocholate into the biliopancreatic duct of Sprague-Dawley rats. The plasma and samples of multiple organ tissues of rats were collected at 3, 6 and 12 h after modeling. The ascites volume, ascites/body weight ratio, and contents of amylase, endotoxin, endothelin-1 (ET-1), nitrogen monoxidum (NO), phospholipase A2 (PLA2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) in plasma were determined. The histological changes of multiple organs were observed under light microscope.

RESULTS: The ascites volume, ascites/body weight ratio, and contents of various inflammatory mediators in blood were higher in the model group than in the sham operation group at all time points [2.38 (1.10), 2.58 (0.70), 2.54 (0.71) vs 0.20 (0.04), 0.30 (0.30), 0.22 (0.10) at 3, 6 and 12 h in ascites/body weight ratio; 1582 (284), 1769 (362), 1618 (302) (U/L) vs 5303 (1373), 6276 (1029), 7538 (2934) (U/L) at 3, 6 and 12 h in Amylase; 0.016 (0.005), 0.016 (0.010), 0.014 (0.015) (EU/mL) vs 0.053 (0.029), 0.059 (0.037), 0.060 (0.022) (EU/mL) at 3, 6 and 12 h in Endotoxin; 3.900 (3.200), 4.000 (1.700), 5.300 (3.000) (ng/L) vs 41.438 (37.721), 92.151 (23.119), 65.016 (26.806) (ng/L) at 3, 6 and 12 h in TNF-α, all P < 0.01]. Visible congestion, edema and lamellar necrosis and massive leukocytic infiltration were found in the pancreas of rats of model group. There were also pathological changes of lung, liver, kidney, ileum, lymphonode, thymus, myocardium and brain.

CONCLUSION: This rat model features reliability, convenience and a high achievement ratio. Complicated with multiple organ injury, it is an ideal animal model of SAP.

Keywords: Severe acute pancreatitis; Multiple organs; Injury; Animal model; Rats; Inflammatory mediator