H Pylori
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 7, 2005; 11(1): 104-108
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.104
Eradication of Helicobacter pylori significantly reduced gastric damage in nonsteroidal anti-inflammatory drug-treated Mongolian gerbils
Chun-Chao Chang, Sheng-Hsuan Chen, Gi-Shih Lien, Horng-Yuan Lou, Ching-Ruey Hsieh, Chia-Lang Fang, Shiann Pan
Chun-Chao Chang, Sheng-Hsuan Chen, Gi-Shih Lien, Horng-Yuan Lou, Ching-Ruey Hsieh, Chia-Lang Fang, Shiann Pan, Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital; Digestive Disease Research center, Taipei Medical University, Taipei, Taiwan, China
Author contributions: All authors contributed equally to the work.
Supported by Taipei Medical University Research Foundation, TMU92-AE-B38 and Wu-Wu Tan Research Foundation
Correspondence to: Dr. Chun-Chao Chang, Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital. No. 252, Wu-Hsing Street, Taipei, Taiwan, China. chunchao@tmu.edu.tw
Telephone: +886-2-27372181-3903 Fax: +886-2-27363051
Received: May 25, 2004
Revised: May 28, 2004
Accepted: June 25, 2004
Published online: January 7, 2005
Abstract

AIM: To examine the effect of eradication of Helicobacter pylori prior to usage of NSAIDs, by investigating gastric inflammatory activity, myeloperoxidase (MPO) activity, prostaglandin (PG) E2 synthesis in H pylori-infected, and H pylori-eradicated gerbils followed by administration of indomethacin and rofecoxib.

METHODS: Six-week-old male gerbils were orally inoculated with H pylori. Seven weeks later, anti-H pylori triple therapy and vehicle were given to gerbils respectively and followed by oral indomethacin (2 mg/kg.d) or rofecoxib (10 mg/kg.d) for 2 wk. We examined the area of lesions, gastric inflammatory activity, PGE2 synthesis and MPO activity in the stomach.

RESULTS: In indomethacin and rofecoxib-treated gerbils, the following results were obtained in H pylori-infected group vs H pylori-eradicated group respectively: hyperplasia area of the stomach (mm2): 82.4±9.2 vs 13.9±3.5 (P<0.05), 30.5±5.1 vs 1.3±0.6 (P<0.05); erosion and ulcer area (mm2): 14.4±4.9 vs 0.86±0.5 (P<0.05), 1.3±0.6 vs 0.4±0.3 (P<0.05); score of gastritis: 7.0±0.0 vs 3.6±0.5 (P<0.05), 7.0±0.0 vs 2.7±0.5 (P<0.05); MPO activity (μmol H2O2/min/g tissue): 104.7±9.2 vs 9.0±2.3 (P<0.05), 133.5±15.0 vs 2.9±0.7 (P<0.05); PGE2 synthesis (pg/mg wet weight/min): 299.2±81.5 vs 102.8±26.2 (P<0.05), 321.4±30.3 vs 11.9±4.8 (P<0.05).

CONCLUSION: Eradication of H pylori reduced gastric damage of NSAID-treated Mongolian gerbils. Rofecoxib caused less severe gastric damage than indomethacin in H pylori-eradicated gerbils.

Keywords: Helicobacter pylori; Gastric damage; NSAIDs