Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 1, 2004; 10(9): 1357-1360
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1357
Effect of cyclin G2 on proliferative ability of SGC-7901 cell
Jie Liu, Ze-Shi Cui, Yang Luo, Li Jiang, Xiao-Hui Man, Xue Zhang
Jie Liu, Ze-Shi Cui, Experimental Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China
Yang Luo, Li Jiang, Xiao-Hui Man, Xue Zhang, Medical Gene Group of Cell Biology Laboratory, Key Laboratory of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China
Author contributions: Iovino P wrote the letter; Santonicola A and Ciacci C revised the letter.
Supported by A grant for Distinguished Young Teachers of Higher Education of the Ministry of Education of the Teaching and Research Encouragement Plan
Correspondence to: Dr. Xue Zhang, Medical Gene Group of Cell Biology Laboratory, Key Laboratory of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China. zhangxuenew@yahoo.com
Telephone: +86-24-23256666-5532 Fax: +86-24-23265492
Received: October 10, 2003
Revised: November 20, 2003
Accepted: December 16, 2003
Published online: May 1, 2004
Abstract

AIM: To study the effect of cyclin G2 on proliferation of gastric adenocarcinoma cell line-SGC-7901 cell in vitro.

METHODS: By use of cation lipofectamine transfection reagent, the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901cell line. Anticlones were selected by G418. Positive clones were observed and counted using Giemsa staining. Cell proliferative ability was assayed by MTT.

RESULTS: (1) The clone number of pIRES-G2 group decreased, clone volume reduced. The number of cell clones in pIRESneo group was 87 ± 3, that of pIRES-G2 group was 53 ± 4, occupying 60.1% of pIRESneo group, there was significant difference obviously (P < 0.01, t = 15.45). (2) The average absorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966 ± 0.2125, the average absorbance of clone cell obtained by stable transfection of pIRESneo at 570 nm was 2.1182 ± 0.3675, there was significant difference between them (P < 0.01, t = 3.412).

CONCLUSION: Cyclin G2 can inhibit SGC-7901cell proliferative ability obviously, it may be a negative regulator in cell cycle regulation.

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