Yoshida N, Nomura K, Matsumoto Y, Nishida K, Wakabayashi N, Konishi H, Mitsufuji S, Kataoka K, Okanoue T, Taniwaki M. Detection of BCL2-IGH rearrangement on paraffin-embedded tissue sections obtained from a small submucosal tumor of the rectum in a patient with recurrent follicular lymphoma. World J Gastroenterol 2004; 10(17): 2602-2604 [PMID: 15300917 DOI: 10.3748/wjg.v10.i17.2602]
Corresponding Author of This Article
Kenichi Nomura, M.D., Ph.D., Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. nomuken@sun.kpu-m.ac.jp
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Case Reports
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World J Gastroenterol. Sep 1, 2004; 10(17): 2602-2604 Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2602
Detection of BCL2-IGH rearrangement on paraffin-embedded tissue sections obtained from a small submucosal tumor of the rectum in a patient with recurrent follicular lymphoma
Naohisa Yoshida, Naoki Wakabayashi, Hideyuki Konishi, Shoji Mitsufuji, Keisho Kataoka, Takeshi Okanoue, Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto 602-8566, Japan
Kenichi Nomura, Yosuke Matsumoto, Kazuhiro Nishida, Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto 602-8566, Japan
Masafumi Taniwaki, Clinical Molecular Genetics and Laboratory Medicine, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto 602-8566, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Kenichi Nomura, M.D., Ph.D., Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. nomuken@sun.kpu-m.ac.jp
Telephone: +81-75-251-5521 Fax: +81-75-251-0710
Received: January 15, 2004 Revised: February 4, 2004 Accepted: February 24, 2004 Published online: September 1, 2004
Abstract
A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma (FL). Colonoscopic examination revealed a rectal submucosal tumor (SMT) without any erosions and ulcers. In this patient, it was difficult to distinguish non-Hodgkin’s lymphoma (NHL) invasion from other disorders of the colon including carcinoid tumor merely based on endoscopic findings. Histopathologic and immunohistochemical studies on biopsy specimens showed an infiltration of atypical lymphocytes that were positive for CD20 and BCL2 but negative for UCHL-1. Fluorescence in situ hybridization on paraffin-embedded tissue sections (T-FISH) identified a translocation of BCL2 with IGH gene. Based on these findings, the tumor was defined as an invasion of FL. T-FISH method is useful for the detection of a monoclonality of atypical lymphocytes in an SMT of the gastrointestinal tract, and particularly for the detection of chromosomal translocations specific to lymphoma subtypes.
