Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 1, 2004; 10(15): 2263-2266
Published online Aug 1, 2004. doi: 10.3748/wjg.v10.i15.2263
In vitro anti-coxsackievirus B3 effect of ethyl acetate extract of Tian-hua-fen
Zhen-Hong Li, Bao-Ming Nie, Hong Chen, Shu-Yun Chen, Ping He, Yang Lu, Xiao-Kui Guo, Jing-Xing Liu
Zhen-Hong Li, Bao-Ming Nie, Hong Chen, Shu-Yun Chen, Ping He, Yang Lu, Xiao-Kui Guo, Jing-Xing Liu, Department of Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China
Author contributions: All authors contributed equally to the work.
Supported by Project of National Nature Science Foundation of China, No. 39970691 and Project from Education Comment of Shanghai, China, No. 2000B04
Correspondence to: Jing-Xing Liu, Department of Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China. lee1217@citiz.net
Telephone: +86-21-64453285 Fax: +86-21-64453285
Received: November 12, 2003
Revised: November 23, 2003
Accepted: February 1, 2004
Published online: August 1, 2004
Abstract

AIM: To investigation the anti-coxsackievirus B3 (CVB3m) effect of the ethyl acetate extract of Tian-hua-fen on HeLa cells infected with CVB3m.

METHODS: HeLa cells were infected with CVB3m and the cytopathic effects (CPE) were observed through light microscope and crystal violet staining on 96-well plate and A600 was detected using spectrophotometer. The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A600.

RESULTS: The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration. Consistent results were got through these two methods. We also investigated the mechanism of its anti-CVB3m effect and the results indicated that the extract represented an inhibitory effect through all the processes of CVB3m attachment, entry, biosynthesis and assemble in cells.

CONCLUSION: The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significant protective effect on HeLa cells infected with CVB3m in a dose-dependent manner and this effect exists through the process of CVB3m attachment, entry, biosynthesis and assemble in cells, suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.

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