Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 1, 2004; 10(15): 2254-2258
Published online Aug 1, 2004. doi: 10.3748/wjg.v10.i15.2254
Pathophysiological significance of a reaction in mouse gastrointestinal tract associated with delayed-type hypersensitivity
Wan-Gui Yu, Ping Lin, Hui Pan, Lan Xiao, En-Cong Gong, Lin Mei
Wan-Gui Yu, Department of Physiology, Medical College of Yangtze University, Jingzhou 434000, Hubei Province, China
Ping Lin, Department of Physiology, Medical School of Hubei Institute for Nationalities, Enshi, 445000, Hubei Province, China
Hui Pan, Lan Xiao, Lin Mei, Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, 100083, China
En-Cong Gong, Department of Pathology, Peking University Health Science Center, Beijing, 100083, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30170419
Correspondence to: Dr. Lin Mei, Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, 100083, China. linmei@bjmu.edu.cn
Telephone: +86-10-82801477 Fax: +86-10-82801746
Received: December 17, 2003
Revised: December 23, 2003
Accepted: January 8, 2004
Published online: August 1, 2004
Abstract

AIM: To explore the pathophysiological significance of delayed type hypersensitivity (DTH) reaction in mouse gastrointestinal tract induced by an allergen 2,4-dinitrochlorobenzene (DNCB).

METHODS: BALB/c mice were randomly divided into control and DTH1-6 groups. After sensitized by DNCB smeared on the abdominal skin, the mice were challenged with DNCB by gavage or enema. The weight, stool viscosity and hematochezia were observed and accumulated as disease active index (DAI) score; the gastrointestinal motility was represented by active charcoal propulsion rate; the colon pathological score was achieved by macropathology and HE staining of section prepared for microscopy; and the leukocyte migration inhibitory factor (LMIF) activity was determined by indirect capillary assay of the absorbance (A) of migrated leukocytes.

RESULTS: Active charcoal propulsion rates of small intestine in the DNCB gavages groups were significantly higher than that in the control group (P < 0.01). The DAI scores and pathological score in DNCB enema groups were also higher than that in the control group ( P < 0.05), and there were significant rises in LMIF activity in DNCB enema groups as compared with control groups (P < 0.01).

CONCLUSION: Mouse gastrointestinal DTH reaction could be induced by DNCB, which might facilitate the mechanism underlying the ulcerative colitis.

Keywords: $[Keywords]