Impact of metabolism-related mutations on the heart rate of gastric cancer patients after peritoneal lavage

Table 1 Validation of the mutation sites in six patients with a sudden drop in heart rate during distilled water lavage

Mutation type	Chr	Start	End	Ref	Alt	Location	Gene name	FOC	Primers for sequencing (5’-3’)
									Forward	Reverse
SNP
1	chr14	20757383	20757383	A	G	UTR3	TTC5	0/6	CCAAAGAAGAGGAAGGACCTAAAG	CTGTCTCTTCCACTGCTCTCTATGTT
2	chr14	20818983	20818983	C	T	intronic	PARP2-920	0/6	TTTATGAGTTGGCATTCAGAAAGTC	GAAAGGTGAAAGGATTTAGGGAAC
3	chr14	20824859	20824859	T	C	intronic	PARP2-156	0/6	ATAAGAGGGAGTCAGAGGAAGGTGT	AGCACTGAGAACATTACTGTATCCCT
4	chr14	20837701	20837701	G	C	exonic	TEP1-886	0/6	GTGTCTTTAGATGTGGTGTTGGCT	TGGCAGAGTCCTCATTTTTGTGT
5	chr14	20841162	20841162	C	T	intronic	TEP1-0948	0/6	CCTCTAACGCCCAGTAAATACACAG	TCTCTTATCCCAGCAGTATCCCAG
6	chr14	20841313	20841313	G	A	intronic	TEP1-0948	0/6
7	chr14	20841707	20841707	C	T	exonic	TEP1-449	0/6	TATTCCGCATACCTTCCCCAC	CCCTGCTCCGTCCCTTCTT
8	chr16	1756178	1756178	T	C	upstream	MAPK8IP3	0/6	GGAAGCGACCACTGCCG	CATCTGGATCTCCATCATCGC
9	chr17	8161149	8161149	C	T	exonic	PFAS	0/6	GAAAGCCTGTGTAACTCTGATTGC	ATTACTGGCTTCAATGGCAACC
10	chr17	73627539	73627539	T	C	exonic	RECQL5	0/6	GCAATGTCCCTGATGTCCTACC	CTGGAGCAAGACCTGCATCG
11	chr21	35470291	35470291	-	T	UTR3	SLC5A3	0/6	CGAATGTGCTTGTGTGATACTTGTT	GGCAGACTTGTTGGTCATTTCAG
12	chr9	98691137	98691137	T	C	exonic	ERCC6L2	1/6	CTTGATTACCGACGACTTGATGG	ATGTTGATAAAGGAAATGAGAGAAAAGC
Indel
13	chr17	8052526	8052532	AAAAACA	-	intronic	PER1	0/6	TGAAACCCCGTCTCTACTGAAAATAC	TCCCTTAGTGGATTCTTCGCAG
14	chr12	133417982	133417982	-	AA	UTR3	CHFR	1/6	GAAAGGCTTCGTCTCTGCTGC	TGGAGGTGAAGAGAGCGTGTTT

Chr: Chromosome number; Ref: Reference sequence; Alt: Alteration sequence; FOC: Frequency of mutation in the control group; SNP: Single nucleotide polymorphism; Indel: Insertion or deletion; TTC5: Tetratricopeptide repeat domain 5; PARP2: Poly (ADP-ribose) polymerase 2; TEP1: Telomerase associated protein 1; MAPK8IP3: Mitogen-activated protein kinase 8 interacting protein 3; PFAS: Phosphoribosylformylglycinamidine synthase; RECQL5: RecQ like helicase 5; SLC5A3: Solute carrier family 5 member 3; ERCC6L2: ERCC excision repair 6 like 2; PER1: Period circadian regulator 1; CHFR: Checkpoint with forkhead and ring finger domains.

Table 2 Baseline clinical information

Characteristics	HR decreased < 30% (n = 172)	HR deceased ≥ 30% (n = 22)	P value
Age (years), mean ± SD	65.6 ± 9.1	65.8 ± 10.4	0.62
Sex, n (%)
Male	72.7	59.1	0.2136
Female	27.3	40.9
Smoking, n (%)	14.0	4.6	0.3190
Drinking, n (%)	7.0	0	0.3668
Hypertension, n (%)	34.3	40.9	0.6361
Diabetes, n (%)	8.1	13.6	0.4171
Hemodynamics before lavage, mean ± SD
Heart rate (bpm)	61 ± 10	62 ± 13	0.511
Diastolic pressure (mmHg)	66 ± 10	65 ± 12	0.6207
Systolic pressure (mmHg)	122 ± 18	119 ± 19	0.3159
Hemodynamics after lavage, mean ± SD
Heart rate (bpm)	56 ± 12	27 ± 20	< 0.0001
Diastolic pressure (mmHg)	76 ± 14	53 ± 23	< 0.0001
Systolic pressure (mmHg)	141 ± 24	92 ± 48	< 0.0001
Change in heart rate (%), mean ± SD	8.3 ± 12.4	59.3 ± 27.7	< 0.0001
Change in diastolic pressure (mmHg), mean ± SD	10.0 ± 13.1	-11.8 ± 18.9	< 0.0001
Change in systolic pressure (mmHg), mean ± SD	17.5 ± 21.8	-26.0 ± 41.5	< 0.0001

HR: Heart rate; SD: Standard deviation.

Table 3 Allele frequency and genotype frequency in the two groups

Characteristics	HR decreased ≥ 30% (n = 22)	HR decreased < 30% (n = 172)	Fisher's P value
TEP1 (rs938886), n (%)
C	14 (0.32)	105 (0.31)	0.8608
G	30 (0.68)	239 (0.70)
CC	1 (0.05)	16 (0.09)	0.5022
CG	12 (0.55)	73 (0.42)
GG	9 (0.41)	83 (0.48)
HWE	0.2278	0.9929
TEP1 (rs1713449), n (%)
C	30 (0.68)	236 (0.69)	0.9546
T	14 (0.32)	108 (0.31)
CC	9 (0.41)	82 (0.48)	0.4465
CT	12 (0.55)	72 (0.42)
TT	1 (0.05)	18 (0.11)
HWE	0.2278	0.7110
RECQL5 (rs820196), n (%)
C	16 (0.36)	109 (0.32)	0.5319
T	28 (0.64)	235 (0.68)
CC	1 (0.05)	19 (0.11)	0.1292
CT	14 (0.64)	71 (0.41)
TT	7 (0.32)	82 (0.48)
HWE	0.0787	0.5420

HR: Heart rate; HWE: Fisher’s P value of Hardy-Weinberg equilibrium test.

Table 4 Haplotype analysis

Items, n (%)	HR decreased ≥ 30% (n = 22)	HR decreased < 30% (n = 172)	Fisher's P value	Odds ratio (95%CI)
CTC	0.00 (0.00)	40.98 (0.12)	0.025601	0.000 (0.000-0.008)
CTT	14.00 (0.32)	64.02 (0.19)	0.042935	2.018 (1.012-4.025)
GCC	16.00 (0.36)	68.02 (0.20)	0.013161	2.293 (1.174-4.477)
GCT	14.00 (0.32)	167.98 (0.49)	0.029281	0.481 (0.246-0.939)
GTT	0.00 (0.00)	3.00 (0.01)	-	-

All frequencies < 0.03 were ignored in the analysis. Loci selected for the hap-analysis: TEP1 (rs938886), TEP1 (rs1713449), and RECQL5 (rs820196). HR: Heart rate; CI: Confidence interval.