Metachronous pulmonary and pancreatic metastases arising from sigmoid colon cancer: A case report

Table 1 Targeted next-generation sequencing of 520 cancer-related genes revealed highly concordant mutation profiles of specimens from the primary sigmoid cancer, lung metastasis, and pancreatic resection

Mutated gene and impact on protein sequence	Primary sigmoid cancer	Lung metastasis	Pancreatic resection
KRAS p.G12A	+	+	+
TP53 p.R248W	+	+	+
TP53 p.C275Y	+	+	+
APC p.E893*	+	+	+
APC p.L1488fs	+	+	+
PTEN p.T319fs	+	+	+
FBXW7 p.E664*	+	+	+
FBXW7 p.G597R	+	+	+
PIK3C2G p.V508A	+	+	+
LRP1B p.A3643D	+	+	+
PIK3CA p.G118D	+
ERCC5 p.T105M	+
FAT3 p.S1086G		+	+
TSHR p.D487N		+	+
ASXL1 p.G668S			+
INHBA p.K198N			+
FGF3 p.A87S		+
INPP4B p.K891M		+
MITF p.I220L		+
EZH2 p.D730fs		+
LRP1B p.P1191T		+
GNAS amplification	+
DAXX amplification		+

+: Tested positive for indicated alteration; APC: APC regulator of WNT signaling pathway; ASXL1: ASXL transcriptional regulator 1; DAXX: Death domain associated protein; ERCC5: ERCC excision repair 5, endonuclease; EZH2: Enhancer of zeste 2 polycomb repressive complex 2 subunit; FAT3: FAT atypical cadherin 3; FBXW7: F-box and WD repeat domain containing 7; FGF3: Fibroblast growth factor 3; GNAS: GNAS complex locus; INHBA: Inhibin subunit beta A; INPP4B: Inositol polyphosphate-4-phosphatase type II B; KRAS: KRAS proto-oncogene, GTPase; LRP1B: LDL receptor related protein 1B; MITF: Melanocyte inducing transcription factor; PIK3C2G: Phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma; PIK3CA: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; PTEN: Phosphatase and tensin homolog; TP53: Tumor protein p53; TSHR: Thyroid stimulating hormone receptor.