Correlations between microbial communities in stool and clinical indicators in patients with metabolic syndrome

Table 1 Detailed clinical data of two groups (mean±SD)

	Group C	Goup D
Gender (male:female)	18:2	12:4
Age	46.70 ± 14.47	47.25 ± 16.34
Weight (kg)	83.63 ± 12.26	68.82 ± 10.32
BMI	28.66 ± 4.12	26.45 ± 3.11
Abdominal circumference (cm)	102.85 ± 10.94	89.64 ± 8.34
Blood pressure (mmhg)	139.60 ± 21.41/81.90 ± 7.75	127.44 ± 16.74/74.63 ± 6.46
Total bilirubin (μmol/L)	17.67 ± 8.67	15.26 ± 7.34
Indirect bilirubin (μmol/L)	13.26 ± 6.89	9.46 ± 4.72
Direct bilirubin (μmol/L)	4.64 ± 3.37	3.52 ± 3.13
Fasting blood sugar (mmol/L)	6.77 ± 1.48	5.22 ± 1.31
Postprandial blood sugar (mmol/L)	11.20 ± 9.83	6.35 ± 2.64
Total protein (g/L)	69.02 ± 6.52	73.46 ± 8.36
Globulin (g/L)	27.09 ± 4.59	32.64 ± 3.56
Albumin (g/L)	42.41 ± 5.88	51.14 ± 6.23
ALB/GLB	1.63 ± 0.39	1.78 ± 0.23
Aspartate transaminase (U/L)	39.57 ± 23.16	31.23 ± 13.54
Alanine aminotransferase (U/L)	50.44 ± 26.82	37.26 ± 14.46
Glutamyl transpeptidase (U/L)	146.13 ± 155.94	36.23 ± 13.63
AST/ALT	0.86 ± 0.39	1.21 ± 0.23
Low density lipoprotein (mmol/L)	3.06 ± 0.57	2.45 ± 0.24
High density lipoprotein (mmol/L)	1.20 ± 0.37	1.65 ± 0.28
LDL/HDL	2.38 ± 1.11	1.98 ± 2.1
Creatinine (μmol/L)	83.54 ± 23.39	73.83 ± 16.34
Triglyceride (mmol/L)	5.66 ± 8.20	1.53 ± 1.25
Total cholesterol (mmol/L)	5.60 ± 3.15	4.76 ± 2.74
Alkaline phosphatase (U/L)	52.46 ± 12.95	89.32 ± 25.32
Urea nitrogen (mmol/L)	6.04 ± 2.12	5.14 ± 2.16
Uric acid (μmol/L)	436.77 ± 141.57	378.35 ± 89.34

BMI: Body mass index; ALB/GLB: Aspartate transaminase/alanine aminotransferase; AST/ALT: Aspartate transaminase/alanine aminotransferase; LDL/HDL: High density lipoprotein/low density lipoprotein.

Table 2 Primers of N701-N712 and S501-S508

Primer name	Primer sequence (5'-3')
N701	CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGG
N702	CAAGCAGAAGACGGCATACGAGATCTAGTACGGTCTCGTGGGCTCGG
N703	CAAGCAGAAGACGGCATACGAGATTTCTGCCTGTCTCGTGGGCTCGG
N704	CAAGCAGAAGACGGCATACGAGATGCTCAGGAGTCTCGTGGGCTCGG
N705	CAAGCAGAAGACGGCATACGAGATAGGAGTCCGTCTCGTGGGCTCGG
N706	CAAGCAGAAGACGGCATACGAGATCATGCCTAGTCTCGTGGGCTCGG
N707	CAAGCAGAAGACGGCATACGAGATGTAGAGAGGTCTCGTGGGCTCGG
N708	CAAGCAGAAGACGGCATACGAGATCCTCTCTGGTCTCGTGGGCTCGG
N709	CAAGCAGAAGACGGCATACGAGATAGCGTAGCGTCTCGTGGGCTCGG
N710	CAAGCAGAAGACGGCATACGAGATCAGCCTCGGTCTCGTGGGCTCGG
N711	CAAGCAGAAGACGGCATACGAGATTGCCTCTTGTCTCGTGGGCTCGG
N712	CAAGCAGAAGACGGCATACGAGATTCCTCTACGTCTCGTGGGCTCGG
S501	AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC
S502	AATGATACGGCGACCACCGAGATCTACACCTCTCTATTCGTCGGCAGCGTC
S503	AATGATACGGCGACCACCGAGATCTACACTATCCTCTTCGTCGGCAGCGTC
S504	AATGATACGGCGACCACCGAGATCTACACAGAGTAGATCGTCGGCAGCGTC
S505	AATGATACGGCGACCACCGAGATCTACACGTAAGGAGTCGTCGGCAGCGTC
S506	AATGATACGGCGACCACCGAGATCTACACACTGCATATCGTCGGCAGCGTC
S507	AATGATACGGCGACCACCGAGATCTACACAAGGAGTATCGTCGGCAGCGTC
S508	AATGATACGGCGACCACCGAGATCTACACCTAAGCCTTCGTCGGCAGCGTC

Table 3 α diversity analysis data of two groups

	Group C	Group D	P value
ACE	2935.84	4496.51	< 0.05
Simpson	0.96	0.93	< 0.05
Shannon	6.98	6.59	> 0.05

ACE index: Used to assess species abundance, the larger the value, the higher the abundance; Simpson index: Used to assess species diversity, the larger the value, the higher the diversity; Shannon index: Used to assess species diversity, the larger the value, the lower the diversity.

Table 4 Relative abundance of top 9 genus in two groups and total samples

Genus	Group C (%)	Group D (%)	Total samples (%)
Prevotella	21.85	31.8	27.38
Bacteroides	19.54	16.34	17.77
Faecalibacterium	7.84	5.54	6.56
Bifidobacterium	2.28	3.18	2.78
Fusobacterium	1.66	3.12	2.47
Ruminococcus	3.48	1.5	2.38
Coprococcus	2.29	1.9	2.07
Lactobacillus	1.76	1.74	1.75
Blautia	1.84	1.5	1.65

The top 9 genus was ranked according to the relative abundance in total samples, and their relative abundance in group C and group D was showed respectively.