Establishment and performance analysis of a new multiplex detection method for influenza an and B virus antigen

Table 1 Characterization parameters of microsphere coupling

	Microsphere recovery, %	MEFL value	CV, %
BAL1, BAL2	> 80	2 × 105-6 × 105	< 25
Quantum dot microspheres	> 80	1 × 106-1.5 × 106	< 25

BAL1: B-aggressive lymphoma 1; BAL2: B-aggressive lymphoma 2; MEFL: Molecules of equivalent fluorescein; CV: Coefficient of variation.

Table 2 Cross-reactivity results of influenza A and B antigens detection

Microspheres	Proteins concentration (pg/mL)		Cross-reaction rate, %
	Influenza B NP proteins	Influenza A NP proteins
Influenza A	2 × 105		0.01
	1 × 106		0.01
Influenza B		2 × 105	0.21
		1 × 106	0.16

NP: Nucleoprotein.

Table 3 Comparison of the results of the multiple detection of influenza A and B antigens and polymerase chain reaction influenza A and B nucleic acid detection results

Antigen multiplexing detection	q-PCR assay		Sensitivity, %	Idiosyncrasyy, %
	Positive	Negative
Influenza A virus
Positive	31	0	57.4	100
Negative	23	27
Influenza B virus
Positive	1	0	100	100
Negative	0	80

q-PCR: Quantitative polymerase chain reaction.