Multinucleated giant cells of bladder mucosa are modified telocytes: Diagnostic and immunohistochemistry algorithm and relation to PD-L1 expression score

Table 1 Antibodies used in the immunohistochemical examination

Monoclonal antibodies	Suppliers	Dilutions
CD31	Dako	1:50
CD34	Dako	1:50
CD34 blasts	Dako	1:100
CD10	Dako	1:50
CD68	Dako	1:100
CD45	Dako	1:300
CD1A	Dako	1:300
P-S100	Dako	1:200
CD117 (C-KIT)	Dako	1:100
P16	Dako	1:25
Smooth muscle actine	Dako	1:100
DESMIN	Dako	1:100
VIMENTIN	Dako	1:100
Ki-67	Dako	1:100
CK AE1/AE3	Dako	1:100
IG G4	Dako	1:100
GATA3	Ventana	1:100
PD-L1 (22C3)	Dako	1:50

Table 2 Immunohistochemical-phenotype of multinucleated giant cells in the stroma of the urothelial carcinoma and the surrounding vesical lamina propria

IHC antibodies	Expression
VIMENTIN	+
SMA	+
DESMIN	+
CD34	+
CD68	+
P16	+
CK AE1/AE3	-
CD31	-
PS100	-
C-KIT (CD117)	-
CD34BLAST	-
KI-67	-
IGG	-
PD-L1	-
GATA3	-
CD10	-
CD45	-
CD1A	-

IHC: Immunohistochemical.