Successful surgical treatment of polybacterial gas gangrene confirmed by metagenomic next-generation sequencing detection: A case report

Table 1 List of antimicrobial susceptibility testing results of Proteus vulgaris/penneri

Code	Antimicrobial	Result	MIC
AMC	Amoxicillin/Clavulanic	Sensitivity	≤ 4/2
AMK	Amikacin	Sensitivity	≤ 8
AMP	Ampicillin	Resistance	8
ATM	Aztreonam	Sensitivity	≤ 2
CAZ	Ceftazidime	Sensitivity	≤ 1
CDR	Cefdinir	-	-
CEC	Cefaclor	-	-
CEP	Cephalothin	-	-
CFM	Cefixime	-	-
CFP	Cefoperazone	-	-
CHL	Chloramphenicol	Sensitivity	≤ 4
CID	Cefonicid	-	-
CIN	Cinoxacin	-	-
CIP	Ciprofloxacin	Sensitivity	0.25
CL	Colistin	Resistance	> 2
CMZ	Cefmetazole	-	-
CPR	Cefprozil	-	-
CPX	Cefpodoxime proxetil	-	-
CRB	Carbenicillin	-	-
CRO	Ceftriaxone	-	-
CSL	Cefoperazone/Sulbactam	-	-
CTT	Cefotetan	-	-
CTX	Cefotaxime	Sensitivity	≤ 1
CXM	Cefuroxime	-	-
CZO	Cefazolin	Resistance	> 16
CZX	Ceftizoxime	-	-
DOX	Doxycycline	-	-
ETP	Ertapenem	-	-
FEP	Cefepime	Sensitivity	≤ 2
FLE	Fleroxacin	-	-
FOS	Fosfomycin	-	-
FOX	Cefoxitin	-	-
GAT	Gatifloxacin	-	-
GEN	Gentamicin	Sensitivity	≤ 2
IPM	Imipenem	Sensitivity	≤ 1
KAN	Kanamycin	-	-
LOM	Lomefloxaccin	-	-
LOR	Loracarbef	-	-
LVX	Levofloxacin	Sensitivity	0.5
MAN	Cefamandole	-	-
MEC	Mecillinam	-	-
MEM	Meropenem	Sensitivity	≤ 1
MEZ	Mezlocillin	-	-
MFX	Moxifloxacin	Mediation	4
MNO	Minocycline	-	-
MOX	Moxalactam	-	-
NET	Netilmicin	-	-
NIT	Nitrofurantoin	-	-
NOR	Norfloxacin	-	-
OFX	Ofloxacin	-	-
PIP	Piperacillin	Sensitivity	≤ 4
SAM	Ampicillin-Sulbactam	Sensitivity	≤ 4/2
SSS	Sulfonamides	-	-
STR	Streptomycin	-	-
SXT	Trimethoprim-Sulfamethoxazole	Sensitivity	≤ 0.5/9.5
TCC	Ticarcillin/Clavulanic	-	-
TCY	Tetracycline	Resistance	≤ 2
TIC	Ticarcillin	-	-
TMP	Trimethoprim	-	-
TOB	Tobramycin	-	-
TZP	Piperacillin-Tazobactam	Sensitivity	≤ 4/4

MIC: Minimum inhibitory concentration.

Table 2 List of antimicrobial susceptibility testing results of Staphylococcus haemolyticus

Code	Antimicrobial	Result	MIC
AMC	Amoxicillin/Clavulanic	Resistance	> 4/2
AMP	Ampicillin	Resistance	> 8
AZM	Azithromycin	-	-
CHL	Chloramphenicol	-	-
CIP	Ciprofloxacin	Resistance	> 4
CLI	Clindamycin	-	-
CLR	Clarithromycin	-	-
DAP	Daptomycin	-	-
DOX	Doxycycline	-	-
ERY	Erythromycin	Resistance	> 4
GEN	Gentamicin	Resistance	> 8
LNZ	Linezolid	Sensitivity	1
LVX	Levofloxacin	-	-
MFX	Moxifloxacin	-	-
MUH	Mupirocin	Resistance	> 256
NIT	Nitrofurantoin	Sensitivity	≤ 16
OFX	Ofloxacin	-	-
OXA	Oxacillin	Resistance	> 2
PEN	Penicillin	Resistance	> 0.25
QDA	Quinupristin-Dalfopristin	-	-
RA	Rifampin	Sensitivity	≤ 0.5
RIF	Rifampin
SXT	Trimethoprim-Sulfamethoxazole	Sensitivity	≤ 1/19
TCY	Tetracycline	Resistance	> 8
TEC	Teicoplanin	Sensitivity	4
TLT	Telithromycin	-	-
TMP	Trimethoprim	Sensitivity	2
VAN	Vancomycin	Sensitivity	2

MIC: Minimum inhibitory concentration.

Table 3 List of bacteria detected by metagenomics next-generation sequencing

Type	Genera	Number of sequences	Species	Number of sequences
G+	Staphylococcus	7166	Staphylococcus haemolyticus	6493
			Staphylococcus aureus	88
G-	Vibrio	7026	Vibrio vulnificus	6491
G+	Streptococcus	973	Streptococcus dysgalactiae	664
G-	Fusobacterium	43	Fusobacterium necrophorum	40
G-	Klebsiella	11	Klebsiella aerogenes	10