Case Report Open Access
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World J Clin Cases. Feb 26, 2022; 10(6): 2007-2014
Published online Feb 26, 2022. doi: 10.12998/wjcc.v10.i6.2007
Primary duodenal dedifferentiated liposarcoma: A case report and literature review
Nah Ihm Kim, Jong Hee Nam, Yoo Duk Choi, Sung Sun Kim, Department of Pathology, Chonnam National University Hospital, Gwangju 61469, South Korea
Ji Shin Lee, Chan Choi, Department of Pathology, Chonnam National University Hwasun Hospital, Hwasun 58128, South Korea
Hee Joon Kim, Department of Surgery, Chonnam National University Hospital, Gwangju 61469, South Korea
ORCID number: Nah Ihm Kim (0000-0001-6215-8549); Ji Shin Lee (0000-0003-4634-2228); Chan Choi (0000-0002-9411-9568); Jong Hee Nam (0000-0001-5991-7102); Yoo Duk Choi (0000-0002-4385-1759); Hee Joon Kim (0000-0002-8636-5726); Sung Sun Kim (0000-0002-8569-2178).
Author contributions: Kim NI, Choi YD, and Kim SS conceptualized the manuscript; reviewed the literature; and interpreted the H&E slides, immunohistochemistry slides, and fluorescence in situ hybridization; Lee JS, Choi C, and Nam JH contributed to the manuscript draft; Kim HJ contributed to the operative performance; all authors read and approved the final manuscript.
Supported by Grant from Chonnam National University Hospital Biomedical Research Institute, No. CRI17004-1.
Informed consent statement: Written informed consent was obtained from the patient for publication of this case report and any accompanying images.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
CARE Checklist (2016) statement: The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sung Sun Kim, MD, PhD, Associate Professor, Department of Pathology, Chonnam National University Hospital, No. 42 Jebong-ro, Gwangju 61469, South Korea. succeedsoon@hanmail.net
Received: September 1, 2021
Peer-review started: September 1, 2021
First decision: October 18, 2021
Revised: October 27, 2021
Accepted: January 11, 2022
Article in press: January 11, 2022
Published online: February 26, 2022
Processing time: 175 Days and 7.2 Hours

Abstract
BACKGROUND

Dedifferentiated liposarcoma (DDLPS) is an extremely rare neoplasm that exhibits various morphologies. The tumor is characterized by immunoreactivity to MDM2 and CDK4 and can be confirmed by detecting MDM2 amplification via fluorescence in situ hybridization (FISH). Herein, we report an unusual case of DDLPS arising from the duodenum.

CASE SUMMARY

A 64-year-old man presented with repeated abdominal pain and weight loss. Radiologic studies revealed a mass of the duodenum involving the pancreas. The patient was treated with pylorus-preserving pancreaticoduodenectomy. Histologically, the tumor showed a high-grade sarcoma. Immunohistochemistry demonstrated that the tumor cells were positive for MDM2 and CDK4 expression. MDM2 amplification was detected via FISH, leading to the final diagnosis of DDLPS. Following surgery, the patient was treated in the intensive care unit due to peritonitis, and died 60 d after surgery.

CONCLUSION

To the best of the authors’ knowledge, this is the first case of primary duodenal DDLPS in Korea and the third case in the English-language literature. Care must be taken not to misdiagnose DDLPS as another high-grade tumor. Liposarcoma should be in the differential diagnosis list.

Key Words: Liposarcoma; Primary; Small bowel; Duodenum; Immunohistochemistry; Case report

Core Tip: Primary dedifferentiated liposarcoma (DDLPS) originating from the duodenum are rare and can be diagnosed based on histology, immunohistochemistry and MDM2 amplification via fluorescence in situ hybridization. Differential diagnoses are required along with consideration of DDLPS.



INTRODUCTION

Liposarcoma is the most common form of malignant soft tissue tumor and tends to occur in the retroperitoneum, deep soft tissues of the trunk, and extremities[1]. Primary liposarcoma of the gastrointestinal tract is extremely rare, with only a few cases reported in the literature[2-10]. In this report, we describe a unique case of dedifferentiated liposarcoma (DDLPS) originating from the duodenum and review previously reported cases. Pathologists should keep liposarcoma in the differential diagnosis list.

CASE PRESENTATION
Chief complaints

A 64-year-old male presented with a 7-d duration of repeated abdominal pain.

History of present illness

The patient also complained of 8 kg weight loss in 3 mo.

History of past illness

The patient had been on antihypertensive medication for 20 years.

Personal and family history

He had no other significant personal or family medical history.

Physical examination

Physical examination revealed diffuse abdominal tenderness, but a palpable mass was not detected.

Laboratory examinations

Laboratory findings showed slight elevation of aspartate aminotransferase (55 U/L). Tumor markers including carcinoembryonic antigen (2.13 mg/mL), were within the normal range.

Imaging examinations

Abdominal computed tomography (CT) revealed a 3 cm-sized heterogeneously enhancing mass in the pancreaticoduodenal groove, causing the obstruction of the second portion of the duodenum.

FINAL DIAGNOSIS

The preoperative differential diagnosis was duodenal adenocarcinoma, gastro-intestinal stromal tumor (GIST), or leiomyosarcoma (LMS). An accurate diagnosis via preoperative upper endoscopy was not possible because only the mucosal surface was collected due to duodenal stenosis. The duodenal tumor was diagnosed as DDLPS by combining all clinical, radiologic, and intraoperative findings and histologic data (Figure 1 and Figure 2).

Figure 1
Figure 1 Radiologic findings. A, B: Abdominal computed tomography scan demonstrated a heterogeneously enhanced mass in the pancreaticoduodenal groove with duodenal obstruction.
Figure 2
Figure 2 Features of tumor cells. A: The tumor was located in the submucosal layer of the duodenum (Hematoxylin-and-eosin stain, ×20); B: At a higher magnification, undifferentiated tumor cells were shown to have marked nuclear atypia with brisk mitotic activity (Hematoxylin-and-eosin stain, ×200); C: Immunohistochemistry revealed positivity for MDM2 in the tumor cells (Immunohistochemistry, ×200); D: MDM2 amplification was detected by MDM2/CEN12 fluorescence in situ hybridization assay (MDM2-green signals, CEN12-red signals, ×1000).
TREATMENT

The patient underwent pylorus-preserving pancreaticoduodenectomy with en bloc right hemicolectomy, superior mesenteric vein segmental resection, and inferior vena cava wedge resection. Intraoperatively, the duodenal mass invaded the pancreas and hepatic flexure of the colon, resulting in gastric and duodenal distension. The tumor also appeared to invade adjacent large blood vessels. Although the duodenal mass was surgically removed, the entire tumor could not be completely removed as the tumor had already spread throughout the body.

Upon macroscopic examination, the surgical resection specimen appeared to have originated from the submucosal layer of the duodenum, measuring 3x3 cm at its greatest dimension. The nodular mass showed a white-to-tan colored cut surface with a focal area of hemorrhage. Pathologic evaluation revealed a tumor arising from the duodenum and extending to the pancreas, colon, and omentum. Histology showed the epicenter of the tumor was the submucosal layer with normal-looking mucosa. The tumor was relatively well circumscribed and composed of high-grade pleomorphic cells. The tumor was arranged in a haphazard, fascicular growth pattern with telangiectatic-like feature. The majority of the tumor cells exhibited marked nuclear atypia with brisk mitotic activity. Undifferentiated tumor cells displayed large vesicular or hyperchromatic nuclei and prominent macronucleoli. Discohesive polygonal giant cells with abundant eosinophilic cytoplasm were also observed. Immunohistochemistry was positive for MDM2 and CDK4 but was negative for CK, Actin, Desmin, CD117, CD34, ERG, S100, TFE3, Melan A, and HMB45. The tumor was also found to harbor MDM2 amplification via fluorescence in situ hybridization (FISH).

OUTCOME AND FOLLOW-UP

Subsequently, chest CT and whole-body positron emission tomography/CT scans were performed to check for preexisting and unidentified intraabdominal liposarcoma outside the gastrointestinal tract and secondary duodenal involvement. No specific abnormalities or metastases were found.

However, due to the vessel invasion of the tumor, cancer seeding contributed to anastomotic dehiscence. Seven days after initial surgery, a 5 mm-sized leakage of the hepaticojejunal anastomosis occurred, leading to generalized peritonitis. The patient underwent a second operation. Large amounts of necrotic fluid had filled the abdominal cavity, and severe adhesions were observed. Hepaticojejunal anastomosis repair with superior mesenteric vein thrombectomy was performed. Fourteen days later, the patient developed sepsis due to the perforation of the gastrojejunal-anastomosis. A life saving emergency operation was performed. Intraoperatively, massive hematoma and bowel adhesion were observed in the upper abdomen. Overall, the tissue was very friable and edematous due to severe inflammation. An external stent was inserted and repaired using a T-tube in the gastrojejunal perforation site. Since the possibility of perforation was very high in the case of primary repair of the jejunal limb, an external stent was inserted using a hemovac drain. After massive irrigation, the operation was terminated.

Unfortunately, the patient did not recover from disseminated intravascular coagulation and peritonitis, and he died 60 d after surgery.

DISCUSSION

Liposarcomas are subclassified as atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDLPS), DDLPS, myxoid liposarcoma, pleomorphic liposarcoma, or myxoid pleomorphic liposarcoma according to the World Health Organization classification[11]. WDLPS is a typically indolent histologic subtype that presents as slowly growing masses but can be locally aggressive with minimal to no distant metastatic potential, while DDLPS is a higher grade histology with the potential for rapid growth and distant metastatic potential[12,13].

The term DDLPS was first introduced by Evans in 1979[14] and is defined as a combination of ALT/WDLPS and a high-grade non-lipogenic sarcoma-like component of variable histologic grade. DDLPS can occur de novo (90%), with about 10% occurring from a pre-existing WDLPS[15]. The histologic hallmark of DDLPS is the transition from ALT/WDLPS to non-lipogenic sarcoma, although a well-differentiated component may not be identifiable.

The tumor usually presents as a large painless mass in late adult life with an equal distribution between males and females. The retroperitoneum is the most common location and occurrence in the extremities and subcutaneous tissue is very rare[16]. Since DDLPS primarily involving the intestine is extremely unusual, less than 10 cases have been reported to date[1-3,5-10,17]. Of those tumors arising in the small bowel, four originated in the jejunum, five in the ileum, and two in the duodenum (Table 1). Clinical information on the precise locations of the other three small bowel tumors is not available. The literature reveals that occurrences of DDLPS in the small intestine can cause various symptoms, such as intussusception, bleeding, obstruction, and abdominal discomfort. Since Okabayashi et al[6] reported the first case in 2013, there has only been one additional report of DDLPS in the duodenum[10].

Table 1 Clinicopathologic features of liposarcoma from small bowel described previous and in present reports.
No.
Ref.
Age (yr)
Sex
Clinical presentation
Location
Histology
Treatment
1Atik et al[2]58FIntussusceptionJejunumLPSNot stated
2Papadopoulos et al[7]52MAbdominal discomfort, vomitingJejunumWDLPSSmall bowel resection
3Rivkind et al[9]UnknownUnknownMimicking appendicitisSmall intestineMyxoid LPSUnknown
4Benaragama et al[3]76MSmall bowel perforationIliac fossaWDLPSSegmental resection
5Patel et al[8]59MPalpable lumpIliac fossaDDLPS with WD componentResection
6Jeong et al[30]45MAbdominal discomfortJejunumDDLPS with WD componentExcision
7Okabayashi et al[6]55MMelenaDuodenumDDLPS with WD componentPancreaticoduodenectomy with partial colon resection
8Nennstiel et al[17]60MGastrointestinal bleedingIleocecal valvePleomorphic LPSIleum segmental resection
9Matsuo et al[5]84MIntussusceptionIleumDDLPSIleocecal resection
10Gajzer et al[1]51MNot providedSmall intestineDDLPS with myofibiorblastic differentiationExcision
11Gajzer et al[1]75MIntestinal obstructionIleumDDLPS with WD componentSegmental ileectomy
12Gajzer et al[1]53MIntestinal obstructionJejunumDDLPSSegmental jejunectomy
13Gajzer et al[1]68MNot providedSmall intestineDDLPS with WD componentExcision
14Whitham et al[10]59FFatigue, palpitation, shortness of breathDuodenum DDLPSSegmental duodenal resection and distal gastrectomy
15Present case64MAbdominal pain,weight lossDuodenumDDLPSPylorus-preserving pancreaticoduodenectomy

Microscopically, the tumor exhibits variable histologic features but mostly undifferentiated pleomorphic cells with striking nuclear atypia. Such tumors with unusual locations and histopathological features may pose a diagnostic challenge. Thus, sarcomatoid carcinoma, GIST, LMS, malignant melanoma and other high-grade sarcomas should be among the list of differential diagnoses.

Sarcomatoid carcinomas should be considered as the primary differential diagnosis. These tumors are predominantly composed of poorly differentiated spindle cells and/or undifferentiated bizarre anaplastic cells resembling fibrosarcoma or LMS. Sarcomatoid carcinomas can be diagnosed by immunohistochemistry using cytokeratin to demonstrate epithelial derivation.

GIST may occur anywhere in the gastrointestinal tract with 30% arising in the small bowel, including the duodenum. The tumor also exhibits a broad morphologic spectrum. While most instances of GIST are spindle or epithelioid cell tumors, progression to high-grade sarcomatous morphology can be seen rarely. The majority of GISTs show expression of CD117, DOG1 and CD34, which may be helpful for diagnosis.

Gastrointestinal LMS is also very rare, with fewer than 100 cases reported in the English-language literature since 2000[18-21]. Typical LMS shows spindle cells with blunt-ended nuclei and eosinophilic fibrillary cytoplasm. The tumor cells are arranged in intersecting fascicles with varying degree of nuclear atypia, necrosis, and brisk mitotic activity. LMS can exhibit a poorly differentiated, pleomorphic appearance in addition to typical areas; this is known as pleomorphic LMS or dedifferentiated LMS[22,23]. For this diagnosis to be established, morphological features characteristic of classic LMS must be present, and are usually positive for at least one myogenic marker, although staining is often weaker and more focal than in typical leiomyosarcomatous areas[22,23]. The diagnosis of LMS should be made on the basis of immunohistochemical stains along with the appropriate morphological features.

Most melanomas of the gastrointestinal tract are metastases from the skin, and primary small bowel melanoma of duodenal origin is extremely rare[24,25]. Because malignant melanomas display various histologic appearance, strong clinical suspicion and precise evaluation are needed to diagnose primary duodenal melanoma.

Based on the morphology of tumor cells in DDLPS, other types of high-grade tumors such as undifferentiated pleomorphic sarcoma, malignant peripheral nerve sheath tumor, and angiosarcoma should be included among the differential diagnoses. The tumor in the present case showed positive immunoreactivity for MDM2 and CDK4. DDLPS was diagnosed based on the histological and immunohistological findings combined with MDM2 amplification via FISH.

Dual staining with MDM2 and CDK4 has been shown to be both sensitive and specific to DDLPS[26]. This is a result of the overexpression of the protein product from chromosomal amplification in the 12q13–15 region of the MDM2 and CDK4 oncogenes. Amplification of these genes can then be confirmed with FISH if diagnostic uncertainty remains[27,28].

However, MDM2 positivity by immunohistochemistry is not a specific indicator of MDM2 amplification because MDM2 positivity is observed in many other sarcomas, including WDLPS, DDLPS, intimal sarcoma, LMS, angiosarcoma, and myxofibro-sarcoma[29]. Validation of MDM2 amplification by FISH, which is currently a gold standard, is mandatory to confirm the diagnosis of DDLPS.

While the biologic behavior of DDLPS appears to be unfavorable, the most effective treatment modality is surgical resection. There have been no published studies of adjuvant therapy due to the paucity of intestinal DDLPS. Because there are only a limited number of cases, we cannot predict the outcomes for DDLPS arising in the small bowel, but we expect similar prognoses compared with soft tissue DDLPS.

CONCLUSION

We presented a unique case of DDLPS originating from the duodenum, one of the rarest locations for gastrointestinal sarcomas. DDLPS should be thoroughly distinguished from its morphological mimickers, as the tumor may be more highly aggressive and extensive than clinically and radiologically expected. While histopathologic features and immunohistochemistry offer evidence of DDLPS, MDM2 FISH is essential to confirm the diagnosis. Pathologists should keep DDLPS among the initial histologic differential diagnoses for high-grade tumors of the gastrointestinal tract.

ACKNOWLEDGEMENTS

We are grateful to the patient for allowing us to use his medical records in our case report.

Footnotes

Provenance and peer review: Unsolicited article; Externally peer reviewed.

Peer-review model: Single blind

Specialty type: Oncology

Country/Territory of origin: South Korea

Peer-review report’s scientific quality classification

Grade A (Excellent): A

Grade B (Very good): 0

Grade C (Good): C, C

Grade D (Fair): 0

Grade E (Poor): 0

P-Reviewer: Esch M, Sperti C, Wang XJ S-Editor: Wang JL L-Editor: A P-Editor: Wang JL

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