Discrepancy among microsatellite instability detection methodologies in non-colorectal cancer: Report of 3 cases

Figure 1 Next-generation sequencing result of the cholangiocarcinoma case. EMA: European Medicines Agency; cfDNA: Cell-free DNA; MSI: Microsatellite instability.

Figure 2 Immunohistochemistry, next-generation sequencing, and PCR results of the cervical cancer case. A: MLH1, MSH2, MSH6 and PMS2 immunohistochemistry (× 14.44, 200 µm). Absence of MLH1 and PMS2 staining in tumor epithelium yet positive internal control staining of lymphocytes in the stroma; B: Next-generation sequencing result; and C: Suspicious instability seen in NR27 after PCR analysis.

Figure 3 Next-generation sequencing result of the case with endometrial carcinoma. MSI: Microsatellite instability; NCCN: National Comprehensive Cancer Network.