Isolation of lymphocytes from the human gastric mucosa

doi:10.5662/wjm.v11.i4.199

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World Journal of Methodology

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2222-0682

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Methodol. Jul 20, 2021; 11(4): 199-207
Published online Jul 20, 2021. doi: 10.5662/wjm.v11.i4.199

Isolation of lymphocytes from the human gastric mucosa

Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Hiroyuki Okada

Masaya Iwamuro, Hiroyuki Okada, Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan

Takahide Takahashi, Natsuki Watanabe, Division of Medical Support, Okayama University Hospital, Okayama 700-8558, Japan

Author contributions: Iwamuro M organized the report and drafted the article; Takahashi T and Watanabe N critically revised the article for important intellectual content; Okada H approved the final article.

Conflict-of-interest statement: The authors declare that they have no conflicts of interest associated with this article.

Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

Corresponding author: Masaya Iwamuro, MD, PhD, Assistant Professor, Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-Ku, Okayama 700-8558, Japan. iwamuromasaya@yahoo.co.jp

Received: December 31, 2020
Peer-review started: December 31, 2020
First decision: April 6, 2021
Revised: April 9, 2021
Accepted: July 9, 2021
Article in press: July 9, 2021
Published online: July 20, 2021
Processing time: 199 Days and 16.3 Hours

Core Tip

Core Tip: This review provides an overview of methodologies used to analyze lymphocytes in the stomach. Helicobacter pylori-associated peptic diseases and gastric cancer are two major subjects of research in this field. Previously reported articles were reviewed, focusing on procedures for isolating human gastric mucosal lymphocytes. The history and present trends in analyzing lymphocytes in patients with gastric disease are also summarized.