Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems

Figure 1 shows a comparison of surface marker analysis between lipoaspirate samples and samples isolated using Lipogems^® and Body-Jet^® eco. A: Approximately 68%-70% of live cells are negative for the CD45 expression; B: 90% of the CD45 negative cells are CD90+CD73+; C: CD34-positive at a high percentage and negative for CD105 (Endoglin) expression. Results are representative of at least three independent experiments for each isolation procedure.

Figure 2 Flow cytometry analysis of adipose-derived stromal cells subpopulation. The analysis shown in this figure was carried out on CD90+CD73+ cells, which were negative for CD45 expression, as indicated on top of the density plots. A: The percentage of CD271- and CD34-positive cells in adipose-derived stromal cells isolated by Lipoaspirate, Lipogems^®, and Body-Jet^® eco procedures is reported; B: Cells positive for CD31 and CD34; C: A wide range of coexpression with CD34 is observed. These cells are also HLA-DR-positive; D: Negative for CD271, as indirectly demonstrated. For greater clarity, red boxed regions highlight the endothelial progenitor cells. Results are representative of at least three independent experiments for each isolation procedure.

Figure 3 Flow cytometry analysis of pericytes and preadipocytes. A and B: Analysis of CD34, CD146 and Pref-1 was performed on the CD90+CD73+CD45- cells, as indicated on top of the density plots. Density plots of CD34 vs CD146 and CD34 vs Pref-1, respectively. Pericytes subpopulation (CD90+CD73+CD45- CD34-CD146+) is highlighted by green boxed regions, while preadipocyte subpopulation (CD90+CD73+CD45- CD34+ Pref-1+), characterized by Pref-1 and CD34 co-expression, is highlighted by blue boxed areas; C: Density plots of CD146 vs Pref-1, indicating their partial co-expression. Results are representative of at least three independent experiments for each isolation procedure.