Sciarretta FV, Appierto V, Campisi S, Jeyaraman N, Jeyaraman M. Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems. World J Transl Med 2026; 12(2): 118113 [DOI: 10.5528/wjtm.118113]
Corresponding Author of This Article
Madhan Jeyaraman, MD, PhD, Researcher, Department of Orthopaedics, ACS Medical College and Hospital, Dr MGR Educational and Research Institute, Velappanchavadi, Chennai 600077, Tamil Nadu, India. madhanjeyaraman@gmail.com
Research Domain of This Article
Cell & Tissue Engineering
Article-Type of This Article
research-article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Share the Article
Sciarretta FV, Appierto V, Campisi S, Jeyaraman N, Jeyaraman M. Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems. World J Transl Med 2026; 12(2): 118113 [DOI: 10.5528/wjtm.118113]
Fabio Valerio Sciarretta, Department of Orthopaedics, Nostra Signora della Mercede Clinic, Rome 00198, Italy
Fabio Valerio Sciarretta, Silvana Campisi, Department of Regenerative Medicine, Biomedical Regenerative Academy (ABRI), Rome 00128, Italy
Vittorio Appierto, Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona 60121, Italy
Silvana Campisi, Department of Regenerative Medicine, Artemisia Lab, Rome 00198, Italy
Naveen Jeyaraman, Madhan Jeyaraman, Department of Orthopaedics, ACS Medical College and Hospital, Dr MGR Educational and Research Institute, Chennai 600077, Tamil Nadu, India
Naveen Jeyaraman, Madhan Jeyaraman, Department of Regenerative Medicine, Agathisha Institute of Stemcell and Regenerative Medicine, Chennai 600030, Tamil Nadu, India
Author contributions: Sciarretta FV designed the research; Sciarretta FV, Appierto V, and Campisi S analyzed the articles for performing the research; Sciarretta FV and Jeyaraman M wrote the manuscript; Jeyaraman N and Jeyaraman M finalized the manuscript.
Institutional review board statement: The study was approved by Institutional Ethics Committee, Sri Lalithambigai Medical College and Hospital.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: All authors declare no conflict of interest in publishing the manuscript.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement- checklist of items.
Data sharing statement: All data is contained within the manuscript.
Corresponding author: Madhan Jeyaraman, MD, PhD, Researcher, Department of Orthopaedics, ACS Medical College and Hospital, Dr MGR Educational and Research Institute, Velappanchavadi, Chennai 600077, Tamil Nadu, India. madhanjeyaraman@gmail.com
Received: December 24, 2025 Revised: April 13, 2026 Accepted: April 28, 2026 Published online: July 28, 2026 Processing time: 217 Days and 13.9 Hours
Abstract
BACKGROUND
Adipose-derived stromal cells (ADSCs) are a mixed cell population, which includes, amongst others, mesenchymal stromal cells (MSCs). The first choice of cell isolation is subcutaneous adipose tissue as it is easily accessible, abundant and easily harvested by a less invasive procedure.
AIM
To determine the viability and phenotypic properties of the ADSCs that are treated by two procedures to induce stromal vascular fraction (SVF) by lipoaspirate, to verify that they can be used to ensure safe and efficient repair of articular cartilage defects.
METHODS
Lipoaspirate samples of adipose tissues were taken using various commercially available systems of withdrawal. The cellular components of SFVs in untreated lipoaspirate samples were compared with similar cells obtained with the use of lipoaspirate samples treated with Lipogems® and Body-Jet® eco systems as a method of assessing cell composition and viability. The multicolour flow cytometry (FC) analysis was used to characterize the outputs.
RESULTS
Multicolour FC analysis revealed that SVF is composed of heterogeneous cell populations such as: (1) Adipose stem cells (CD45-CD90+CD73+CD34+ CD31- CD105-CD146-); (2) Endothelial progenitor cells (CD45-CD90+ CD73+CD34+ CD31+ CD105 LowCD146+); and (3) Pericytes (CD45-CD90+CD73+ CD34- CD31-CD105-CD146+), and other more or less characterized cells. The overall cell viability was similar across the groups. However, we observed a steady rise in the percentage of the endothelial cells and pericytes in the processed samples with Lipogems® and Body-Jet® eco systems in comparison to the untreated lipoaspirate samples.
CONCLUSION
Phenotypic characterization studies have suggested that MSC populations are found in a perivascular site with ADSCs shared with pericytes and endothelial cells. We have indications that the mechanical withdrawal steps can leave a large population heterogeneity of cells, retaining a more intricate tissue architecture (niche).
Core Tip: Adipose-derived stromal cells (ADSCs) represent a heterogeneous population with mesenchymal stromal cells, pericytes, and endothelial progenitors. Subcutaneous adipose tissue provides a minimally invasive and abundant source for stromal vascular fraction isolation. A comparative analysis of untreated lipoaspirate vs Lipogems® and Body-Jet® eco systems revealed preserved viability and enhanced endothelial/pericyte proportions. Mechanical processing maintains cellular heterogeneity and niche architecture, thereby supporting the potential for safe and effective articular cartilage repair by ADSCs.
