Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.
World J Transl Med. Jul 28, 2026; 12(2): 118113
Published online Jul 28, 2026. doi: 10.5528/wjtm.118113
Published online Jul 28, 2026. doi: 10.5528/wjtm.118113
Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems
Fabio Valerio Sciarretta, Department of Orthopaedics, Nostra Signora della Mercede Clinic, Rome 00198, Italy
Fabio Valerio Sciarretta, Silvana Campisi, Department of Regenerative Medicine, Biomedical Regenerative Academy (ABRI), Rome 00128, Italy
Vittorio Appierto, Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona 60121, Italy
Silvana Campisi, Department of Regenerative Medicine, Artemisia Lab, Rome 00198, Italy
Naveen Jeyaraman, Madhan Jeyaraman, Department of Orthopaedics, ACS Medical College and Hospital, Dr MGR Educational and Research Institute, Chennai 600077, Tamil Nadu, India
Naveen Jeyaraman, Madhan Jeyaraman, Department of Regenerative Medicine, Agathisha Institute of Stemcell and Regenerative Medicine, Chennai 600030, Tamil Nadu, India
Author contributions: Sciarretta FV designed the research; Sciarretta FV, Appierto V, and Campisi S analyzed the articles for performing the research; Sciarretta FV and Jeyaraman M wrote the manuscript; Jeyaraman N and Jeyaraman M finalized the manuscript.
Institutional review board statement: The study was approved by Institutional Ethics Committee, Sri Lalithambigai Medical College and Hospital.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: All authors declare no conflict of interest in publishing the manuscript.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement- checklist of items.
Data sharing statement: All data is contained within the manuscript.
Corresponding author: Madhan Jeyaraman, MD, PhD, Researcher, Department of Orthopaedics, ACS Medical College and Hospital, Dr MGR Educational and Research Institute, Velappan
Received: December 24, 2025
Revised: April 13, 2026
Accepted: April 28, 2026
Published online: July 28, 2026
Processing time: 217 Days and 13.9 Hours
Revised: April 13, 2026
Accepted: April 28, 2026
Published online: July 28, 2026
Processing time: 217 Days and 13.9 Hours
Core Tip
Core Tip: Adipose-derived stromal cells (ADSCs) represent a heterogeneous population with mesenchymal stromal cells, pericytes, and endothelial progenitors. Subcutaneous adipose tissue provides a minimally invasive and abundant source for stromal vascular fraction isolation. A comparative analysis of untreated lipoaspirate vs Lipogems® and Body-Jet® eco systems revealed preserved viability and enhanced endothelial/pericyte proportions. Mechanical processing maintains cellular heterogeneity and niche architecture, thereby supporting the potential for safe and effective articular cartilage repair by ADSCs.