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©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Nephrol. Sep 25, 2025; 14(3): 108534
Published online Sep 25, 2025. doi: 10.5527/wjn.v14.i3.108534
Published online Sep 25, 2025. doi: 10.5527/wjn.v14.i3.108534
Adipose-derived mesenchymal stromal cell secretome protects against kidney injury through induction of heme oxygenase 1 upregulation in vitro
Hanan Jafar, Nidaa A Ababneh, Renata M Alatoom, Suzan Zalloum, Abdalla Awidi, Cell Therapy Center, The University of Jordan, Amman 11942, Jordan
Dana Alhattab, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
Dana Alhattab, KAUST Center of Excellence for Smart Health, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia
Bareqa Salah, Hussein Alhawari, Abdalla Awidi, School of Medicine, The University of Jordan, Amman 11942, Jordan
Bareqa Salah, Department of General Surgery, Jordan University Hospital, Amman 11942, Jordan
Hussein Alhawari, Abdalla Awidi, Department of Internal Medicine, Jordan University Hospital, Amman 11942, Jordan
Co-corresponding authors: Nidaa A Ababneh and Abdalla Awidi.
Author contributions: Jafar H, Ababneh NA, and Awidi A conceived the study and prepared the methodology; Jafar H and Ababneh NA performed the formal analyses, and wrote the manuscript; Jafar H and Awidi A secured the funding, and reviewed the manuscript; Alhattab D performed data the curation and visualization; Alatoom RM and Zalloum S performed the experiments; Salah B and Alhawari H performed the clinical methods and provided resources; Ababneh NA and Awidi A supervised the work; All authors have read and approved the final manuscript. The two co-corresponding authors made equal contributions to the conception, supervision, and administration of this work. Both authors jointly worked on the experimental design, data interpretation, and manuscript preparation, and will continue to share responsibility for post-publication inquiries.
Supported by Abdul Hameed Shoman Foundation, No. 6/2018.
Institutional review board statement: All subjects had signed written informed consents before participation in the study, which was approved by the Institutional Review Board ( approval number IRB-CTC-1-2022-08) at the Cell Therapy Center adhering to the ethical guidelines of the Declaration of Helsinki.
Conflict-of-interest statement: The authors have no relevant financial or non-financial interests to disclose.
Data sharing statement: All data generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Abdalla Awidi, Cell Therapy Center, The University of Jordan, Queen Rania St, Amman 11942, Amman, Jordan. aabbadi@ju.edu.jo
Received: April 17, 2025
Revised: May 29, 2025
Accepted: July 15, 2025
Published online: September 25, 2025
Processing time: 153 Days and 22.7 Hours
Revised: May 29, 2025
Accepted: July 15, 2025
Published online: September 25, 2025
Processing time: 153 Days and 22.7 Hours
Core Tip
Core Tip: This study highlights the therapeutic potential of secretome derived from adipose-derived mesenchymal stromal cells (ADMSCs) in protecting renal tubular epithelial cells under oxidative stress conditions. The ADMSC secretome reduced reactive oxygen species levels, enhanced mitochondrial membrane potential, and upregulated heme oxygenase 1 gene expression, indicating activation of cytoprotective pathways. Comparative transcriptome analysis with bone marrow-derived mesenchymal stromal cells revealed distinct gene expression profiles related to mitochondrial and antioxidant functions. These findings support the use of ADMSC secretome as a promising cell-free approach for mitigating oxidative damage in acute kidney injury.